Faculty of Dentistry, McGill University, 3640 University Street, Montreal, H3A 2B2 QC, Canada.
Faculty of Dentistry, Universidad Complutense de Madrid, Spain.
Biomaterials. 2014 Jul;35(21):5436-45. doi: 10.1016/j.biomaterials.2014.03.050. Epub 2014 Apr 13.
Onlay grafts made of monolithic microporous monetite bioresorbable bioceramics have the capacity to conduct bone augmentation. However, there is heterogeneity in the graft behaviour in vivo that seems to correlate with the host anatomy. In this study, we sought to investigate the metabolic activity of the regenerated bone in monolithic monetite onlays by using positron emission tomography-computed tomography (PET-CT) in rats. This information was used to optimize the design of monetite onlays with different macroporous architecture that were then fabricated using a 3D-printing technique. In vivo, bone augmentation was attempted with these customized onlays in rabbits. PET-CT findings demonstrated that bone metabolism in the calvarial bone showed higher activity in the inferior and lateral areas of the onlays. Histological observations revealed higher bone volume (up to 47%), less heterogeneity and more implant osseointegration (up to 38%) in the augmented bone with the customized monetite onlays. Our results demonstrated for the first time that it is possible to achieve osseointegration of dental implants in bone augmented with 3D-printed synthetic onlays. It was also observed that designing the macropore geometry according to the bone metabolic activity was a key parameter in increasing the volume of bone augmented within monetite onlays.
整体式微孔单水磷酸氢钙生物可吸收生物陶瓷的骨增量能力。然而，在体内移植物的行为存在异质性，似乎与宿主解剖结构有关。在这项研究中，我们试图通过使用正电子发射断层扫描-计算机断层扫描（PET-CT）在大鼠中研究整体式单水磷酸氢钙覆盖物中再生骨的代谢活性。该信息用于优化不同大孔结构的单水磷酸氢钙覆盖物的设计，然后使用 3D 打印技术制造。在体内，在兔子中尝试用这些定制的覆盖物进行骨增量。PET-CT 研究结果表明，颅骨骨中的骨代谢在覆盖物的下侧和外侧区域显示出更高的活性。组织学观察显示，定制的单水磷酸氢钙覆盖物在增强骨中具有更高的骨体积（高达 47%）、更少的异质性和更多的植入体骨整合（高达 38%）。我们的研究结果首次证明，有可能实现通过 3D 打印合成覆盖物进行骨增量的牙种植体的骨整合。还观察到，根据骨代谢活性设计大孔几何形状是增加单水磷酸氢钙覆盖物内骨增量体积的关键参数。
