Mikula Eric, Hollman Kyle, Chai Dongyul, Jester James V, Juhasz Tibor
Department of Biomedical Engineering, University of California, Irvine, Irvine, California, USA.
Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA; Soundsight Research, Livonia, Michigan, USA.
Ultrasound Med Biol. 2014 Jul;40(7):1671-9. doi: 10.1016/j.ultrasmedbio.2013.11.009. Epub 2014 Apr 13.
To investigate the role of collagen structure in corneal biomechanics, measurement of localized corneal elasticity with minimal destruction to the tissue is necessary. We adopted the recently developed acoustic radiation force elastic microscopy (ARFEM) technique to measure localize biomechanical properties of the human cornea. In ARFEM, a low-frequency, high-intensity acoustic force is used to displace a femtosecond laser-generated microbubble, while high-frequency, low-intensity ultrasound is used to monitor the position of the microbubble within the cornea. Two ex vivo human corneas from a single donor were dehydrated to physiologic thickness, embedded in gelatin and then evaluated using the ARFEM technique. In the direction perpendicular to the corneal surface, ARFEM measurements provided elasticity values of E = 1.39 ± 0.28 kPa for the central anterior cornea and E = 0.71 ± 0.21 kPa for the central posterior cornea in pilot studies. The increased value of corneal elasticity in the anterior cornea correlates with the higher density of interweaving lamellae in this region.
为了研究胶原蛋白结构在角膜生物力学中的作用，有必要在对组织破坏最小的情况下测量局部角膜弹性。我们采用了最近开发的声辐射力弹性显微镜（ARFEM）技术来测量人角膜的局部生物力学特性。在ARFEM中，低频、高强度声力用于移动飞秒激光产生的微泡，而高频、低强度超声用于监测微泡在角膜内的位置。取自单一供体的两片离体人角膜脱水至生理厚度，嵌入明胶中，然后使用ARFEM技术进行评估。在初步研究中，在垂直于角膜表面的方向上，ARFEM测量得出中央前角膜的弹性值为E = 1.39 ± 0.28 kPa，中央后角膜的弹性值为E = 0.71 ± 0.21 kPa。前角膜中角膜弹性值的增加与该区域交织板层的较高密度相关。
