评估果蝇心脏发育、功能和衰老的方法。
Methods to assess Drosophila heart development, function and aging.
作者信息
Ocorr Karen, Vogler Georg, Bodmer Rolf
机构信息
Development, Aging and Regeneration Program, Sanford-Burnham Medical Research Institute, 10901 N. Torrey Pines Rd., La Jolla, CA 92037, United States.
Development, Aging and Regeneration Program, Sanford-Burnham Medical Research Institute, 10901 N. Torrey Pines Rd., La Jolla, CA 92037, United States.
出版信息
Methods. 2014 Jun 15;68(1):265-72. doi: 10.1016/j.ymeth.2014.03.031. Epub 2014 Apr 12.
Abstract
In recent years the Drosophila heart has become an established model for many different aspects of human cardiac disease. This model has allowed identification of disease-causing mechanisms underlying congenital heart disease and cardiomyopathies and has permitted the study of underlying genetic, metabolic and age-related contributions to heart function. In this review we discuss methods currently employed in the analysis of the Drosophila heart structure and function, such as optical methods to infer heart function and performance, electrophysiological and mechanical approaches to characterize cardiac tissue properties, and conclude with histological techniques used in the study of heart development and adult structure.
摘要
近年来，果蝇心脏已成为研究人类心脏病诸多不同方面的既定模型。该模型有助于识别先天性心脏病和心肌病背后的致病机制，并能对影响心脏功能的潜在遗传、代谢及与年龄相关的因素进行研究。在本综述中，我们将讨论目前用于分析果蝇心脏结构和功能的方法，比如推断心脏功能和性能的光学方法、表征心脏组织特性的电生理和机械方法，并以用于研究心脏发育和成年结构的组织学技术作为总结。
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