Figueroa G, Galeno H, Troncoso M, Toledo S, Soto V
Microbiology Unit, University of Chile, Santiago.
J Clin Microbiol. 1989 May;27(5):1040-4. doi: 10.1128/jcm.27.5.1040-1044.1989.
A prospective study of Campylobacter jejuni infection was performed during a 6-month period in a cohort of 198 Chilean infants. Surveillance was based on biweekly home visits by a team of trained nurses. C. jejuni colonization was studied by culturing stool samples from diarrheal episodes and paired asymptomatic controls and by performing an enzyme-linked immunosorbent assay developed to measure C. jejuni outer membrane protein polyvalent antibodies in a representative group of infants. C. jejuni was isolated from 30 of 299 diarrheal episodes (10%) and from 17 of 304 samples from asymptomatic infants (6%). Significantly higher (P less than 0.05) C. jejuni illness/infection ratios were found in infants less than 9 months old. Polyvalent antibodies to C. jejuni were found in 27 of 89 serum samples (30%). The lowest prevalence (4%) was observed among infants less than 9 months old, and the highest was found in patients older than 15 months (63%; P less than 0.01). Data obtained by analysis of paired serum samples revealed a significant increase in the number of seropositive individuals, from 8% on admission to 50% at the end of the protocol (P less than 0.001). The change in the immune status of these infants was associated with symptomatic infection in 9 of 18 cases. The antibody rise found in the remaining nine infants suggested the presence of asymptomatic C. jejuni infections. This inference was documented by the isolation of C. jejuni from stools of two of these infants when the infants were randomly studied while asymptomatic. This study points out the impact of C. jejuni-associated diarrheal episodes and the high frequency with which asymptomatic infections elicit specific antibodies in infants living in areas such as Chile.
在6个月期间，对198名智利婴儿进行了空肠弯曲菌感染的前瞻性研究。监测工作由一组经过培训的护士每两周进行一次家访。通过培养腹泻发作时的粪便样本以及配对的无症状对照样本，并通过进行一种酶联免疫吸附测定法来研究空肠弯曲菌定植情况，该测定法用于测量一组有代表性的婴儿中空肠弯曲菌外膜蛋白多价抗体。在299次腹泻发作中有30次分离出空肠弯曲菌（10%），在304份无症状婴儿样本中有17次分离出空肠弯曲菌（6%）。在9个月以下的婴儿中发现空肠弯曲菌疾病/感染率显著更高（P小于0.05）。在89份血清样本中有27份（30%）发现了空肠弯曲菌多价抗体。在9个月以下的婴儿中患病率最低（4%），在15个月以上的患者中患病率最高（63%；P小于0.01）。对配对血清样本分析获得的数据显示，血清阳性个体数量显著增加，从入院时的8%增至方案结束时的50%（P小于0.001）。这些婴儿免疫状态的变化在18例中有9例与有症状感染相关。其余9名婴儿抗体升高表明存在无症状空肠弯曲菌感染。当对其中两名婴儿在无症状时进行随机研究时，从他们的粪便中分离出空肠弯曲菌，这一推断得到了证实。这项研究指出了空肠弯曲菌相关腹泻发作的影响，以及在智利等地区生活的婴儿中无症状感染引发特异性抗体的高频率。
