在微流控装置中利用电场调节肺癌细胞的趋化性。
Modulating chemotaxis of lung cancer cells by using electric fields in a microfluidic device.
机构信息
Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei 10617, Taiwan ; Research Center for Applied Sciences, Academia Sinica, 11529 Taipei, Taiwan.
Institute of Biophotonics, National Yang-Ming University, 11221 Taipei, Taiwan ; Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang-Ming University, 11221 Taipei, Taiwan.
出版信息
Biomicrofluidics. 2014 Apr 1;8(2):024107. doi: 10.1063/1.4870401. eCollection 2014 Mar.
Abstract
We employed direct-current electric fields (dcEFs) to modulate the chemotaxis of lung cancer cells in a microfluidic cell culture device that incorporates both stable concentration gradients and dcEFs. We found that the chemotaxis induced by a 0.5 μM/mm concentration gradient of epidermal growth factor can be nearly compensated by a 360 mV/mm dcEF. When the effect of chemical stimulation was balanced by the electrical drive, the cells migrated randomly, and the path lengths were largely reduced. We also demonstrated electrically modulated chemotaxis of two types of lung cancer cells with opposite directions of electrotaxis in this device.
摘要
我们在一种微流控细胞培养装置中采用直流电场（dcEFs）来调节肺癌细胞的趋化性，该装置同时包含稳定的浓度梯度和 dcEFs。我们发现，表皮生长因子 0.5 μM/mm 的浓度梯度诱导的趋化性可以被 360 mV/mm 的直流电场几乎完全补偿。当化学刺激的效果被电驱动平衡时，细胞会随机迁移，并且路径长度大大减小。我们还在该装置中证明了两种具有相反电趋性的肺癌细胞的电调制趋化性。
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