果糖饮食和肾衰竭对胰岛功能的影响。

Impact of fructose diet and renal failure on the function of pancreatic islets.

作者信息

Pokrywczynska Marta, Flisinski Mariusz, Jundzill Arkadiusz, Krzyzanowska Sandra, Brymora Andrzej, Deptula Aleksander, Bodnar Magdalena, Kloskowski Tomasz, Stefanska Anna, Marszalek Andrzej, Manitius Jacek, Drewa Tomasz

机构信息

From the Departments of *Tissue Engineering, and †Nephrology, Internal Medicine and Hypertension, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz; ‡Department of Dialysis, New Hospital in Świecie, Świecie; Departments of §Microbiology, ∥Clinical Pathomorphology, and ¶Laboratory Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz; #Department of Pathology, Poznan University of Medical Sciences, Poznan; and **Department of Urology, Nicolaus Copernicus Hospital, Torun, Poland.

出版信息

Pancreas. 2014 Jul;43(5):801-8. doi: 10.1097/MPA.0000000000000111.

DOI:10.1097/MPA.0000000000000111

PMID:24739489

Abstract

OBJECTIVES

This study was designed to evaluate the impact of fructose-rich diet and chronic kidney disease (CKD) on the in vitro function of pancreatic islets.

METHODS

Fifty-four rats were divided into 3 equal groups as follows: control, rats with CKD 1/2 that underwent surgical uninephrectomy, and rats with CKD 5/6 that underwent uninephrectomy and kidney cortex mass resection. Each group was further assigned to 3 diet protocols--regular diet, regular diet with 10% fructose (F10), and 60% fructose-rich diet (F60). After 8 weeks of insulin administration, C-peptide, glycated hemoglobin level, serum urea nitrogen, creatinine clearance, and homeostasis model assessment of insulin resistance were evaluated. Static glucose insulin stimulation test of isolated pancreatic islets and histologic analysis of pancreatic tissue were performed.

RESULTS

The F10 diet increased the levels of insulin and C-peptide in all groups. Homeostasis model assessment of insulin resistance was increased in all animals fed with fructose. The elevated levels of creatinine and diminished creatinine clearance were detected in CKD 5/6 rats fed with 60% fructose-rich diet. The F10 diet resulted in high levels of serum insulin and C-peptide and glucose-stimulated insulin secretion. Fructose-rich diet increased the islet size and number, with irregular morphology and exocrine tissue fibrosis.

CONCLUSIONS

The fructose-rich diet accelerates the progression of CKD and affects the pancreatic islet function.

摘要

目的

本研究旨在评估富含果糖的饮食和慢性肾脏病（CKD）对胰岛体外功能的影响。

方法

54只大鼠被分为3组，每组数量相等，分组如下：对照组、接受单侧肾切除术的CKD 1/2大鼠组，以及接受单侧肾切除术和肾皮质部分切除术的CKD 5/6大鼠组。每组进一步分为3种饮食方案——常规饮食、含10%果糖的常规饮食（F10）和富含60%果糖的饮食（F60）。在给予胰岛素8周后，评估C肽、糖化血红蛋白水平、血清尿素氮、肌酐清除率和胰岛素抵抗的稳态模型评估。对分离的胰岛进行静态葡萄糖胰岛素刺激试验，并对胰腺组织进行组织学分析。

结果

F10饮食使所有组的胰岛素和C肽水平升高。所有喂食果糖的动物胰岛素抵抗的稳态模型评估均升高。在喂食60%富含果糖饮食的CKD 5/6大鼠中检测到肌酐水平升高和肌酐清除率降低。F10饮食导致血清胰岛素、C肽水平升高以及葡萄糖刺激的胰岛素分泌增加。富含果糖的饮食增加了胰岛的大小和数量，形态不规则且外分泌组织纤维化。