Yeh C T, Wei J S, Liaw Y F
Liver Unit, Chang Gung Memorial Hospital at Lin Kou, Republic of China.
Clin Chem. 1989 Aug;35(8):1684-7.
Using chromatography on diethylaminoethyl (DEAE) cellulose, we measured biliary alkaline phosphatase (BALP; EC 3.1.3.1) activities in sera from 182 patients, most with hepatobiliary disorders but some with non-hepatobiliary diseases. Relative BALP activities were extremely low in otherwise healthy carriers of hepatitis B virus (mean: 5.4 U/L) and in patients with non-hepatobiliary diseases (mean: 5.3 U/L). Although BALP activities were detectable in some cases of liver cirrhosis and chronic hepatitis, these values were generally low (respective means: 12.6 and 12.0 U/L). High BALP activities were detected in patients with primary hepatocellular carcinoma, secondary metastatic liver tumors, and obstructive jaundice: mean values were 27.2, 37.2, and 73.6 U/L, respectively. There was no correlation between BALP activity and bilirubin concentration in patients with obstructive jaundice, nor between BALP activities in obstructive jaundice caused by stones and in those caused by extrahepatic tumor. Some patients with primary hepatocellular carcinoma had high BALP but low alpha-fetoprotein (AFP) values, some others the reverse. Based on AFP alone, the sensitivity for detecting hepatocellular carcinoma was 79%; adding BALP in parallel improved the sensitivity to 97%. We found minicolumn chromatography on DEAE-cellulose useful for determining BALP activity in hepatobiliary diseases.
我们采用二乙氨基乙基（DEAE）纤维素柱色谱法，测定了182例患者血清中的胆汁碱性磷酸酶（BALP；EC 3.1.3.1）活性，这些患者大多患有肝胆疾病，但也有一些患有非肝胆疾病。在乙肝病毒健康携带者（均值：5.4 U/L）和非肝胆疾病患者（均值：5.3 U/L）中，相对BALP活性极低。虽然在一些肝硬化和慢性肝炎病例中可检测到BALP活性，但这些值通常较低（各自均值：12.6和12.0 U/L）。在原发性肝细胞癌、继发性肝转移瘤和梗阻性黄疸患者中检测到高BALP活性：均值分别为27.2、37.2和73.6 U/L。梗阻性黄疸患者的BALP活性与胆红素浓度之间无相关性，结石所致梗阻性黄疸和肝外肿瘤所致梗阻性黄疸患者的BALP活性之间也无相关性。一些原发性肝细胞癌患者BALP高但甲胎蛋白（AFP）值低，另一些患者则相反。仅基于AFP，检测肝细胞癌的敏感性为79%；同时加入BALP可将敏感性提高至97%。我们发现DEAE - 纤维素微柱色谱法有助于测定肝胆疾病中的BALP活性。
