a Department of Pharmacy and Biotechnology , University of Bologna , Via Massarenti 9, 40138 Bologna , Italy.
Nat Prod Res. 2014;28(18):1438-45. doi: 10.1080/14786419.2014.909421. Epub 2014 Apr 22.
The aim of this study was to determine the in vitro antimicrobial activity of crude extracts of three plants from Ayurveda tradition (Tinospora cordifolia, Alstonia scholaris, Crataeva nurvala) against reference microbial strains and clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) and carbapenemase-producing Klebsiella pneumoniae. IC50 values were obtained by micro-dilution methods meeting the requirements of the NCCLS standard. The cytotoxicity of the extracts was also investigated on a mammalian cell line. Extracts displayed a variable degree of antimicrobial activity and did not interfere with mammalian cell proliferation. T. cordifolia and A. scholaris exhibited a higher inhibitory activity against clinical isolates of MRSA and carbapenemase-producing K. pneumoniae compared with reference strains, while C. nurvala exhibited a different behaviour. An antifungal activity towards Candida albicans was observed for A. scholaris extract. Results indicate that constituents from T. cordifolia and A. scholaris may be a potential source of new therapeutic strategies for infectious diseases.
本研究旨在确定三种源自印度传统医学（三叶鬼针草、垂榕、窄叶火筒树）的粗提物对耐甲氧西林金黄色葡萄球菌（MRSA）和产碳青霉烯酶肺炎克雷伯菌参考菌株和临床分离株的体外抗菌活性。采用符合 NCCLS 标准要求的微量稀释法测定 IC50 值。还在哺乳动物细胞系上研究了提取物的细胞毒性。提取物表现出不同程度的抗菌活性，且不干扰哺乳动物细胞增殖。三叶鬼针草和垂榕对 MRSA 和产碳青霉烯酶肺炎克雷伯菌的临床分离株的抑制活性高于参考菌株，而窄叶火筒树则表现出不同的行为。垂榕提取物对白色念珠菌具有抗真菌活性。结果表明，三叶鬼针草和垂榕的成分可能是治疗感染性疾病的新治疗策略的潜在来源。
