Hassanin M B, Ghosh L, Das A K, Waterhouse J P
Department of Pathology, University of Illinois, Chicago 60680.
J Oral Pathol Med. 1989 May;18(5):291-8. doi: 10.1111/j.1600-0714.1989.tb00400.x.
The purpose of the present investigation was to study the histogenesis of the mucoepidermoid carcinoma of the salivary glands. Eleven cases of mucoepidermoid carcinoma of the minor salivary glands and five of the major glands were extensively studied employing immunohistochemical and fluorescent microscopic techniques. Both the intermediate cells and the duct cells showed a rather similar pattern of reactivity for vimentin, actin and EMA. Also, the intermediate cells and the myoepithelial cells showed a similar reaction pattern for keratin and UGA-1. The intermediate, myoepithelial and duct cells shared a similar reaction pattern for desmin, myosin, CEA, and S-100 protein. However, the rest of the tumor markers studied (AFP, PNA and WGA) were found to be non contributary. We also found that the intermediate and to some extent the epidermoid tumor cells showed a positive reaction with Azophloxine GA, which is a selective stain for myoepithelial cells in the normal glands. Based on these findings, the duct cells, the myoepithelial cell in the normal glands and the intermediate cells of the mucoepidermoid carcinoma share certain similar characteristics. The intermediate cells may actually be a mixed population, some having characteristics of the myopithelial cells and others of duct cells. These findings are relevant to the possible role of the intermediate cell in the histogenesis of the mucoepidermoid carcinoma.
本研究的目的是探讨涎腺黏液表皮样癌的组织发生。应用免疫组织化学和荧光显微镜技术，对11例小涎腺黏液表皮样癌和5例大涎腺黏液表皮样癌进行了广泛研究。中间细胞和导管细胞对波形蛋白、肌动蛋白和上皮膜抗原显示出相当相似的反应模式。此外，中间细胞和肌上皮细胞对角蛋白和UGA-1显示出相似的反应模式。中间细胞、肌上皮细胞和导管细胞对结蛋白、肌球蛋白、癌胚抗原和S-100蛋白具有相似的反应模式。然而，研究的其他肿瘤标志物（甲胎蛋白、花生凝集素和麦胚凝集素）未发现有作用。我们还发现，中间细胞以及在一定程度上表皮样肿瘤细胞与偶氮荧光桃红GA呈阳性反应，偶氮荧光桃红GA是正常腺体中肌上皮细胞的一种选择性染色剂。基于这些发现，导管细胞、正常腺体中的肌上皮细胞和黏液表皮样癌的中间细胞具有某些相似特征。中间细胞实际上可能是一个混合群体，一些具有肌上皮细胞的特征，另一些具有导管细胞的特征。这些发现与中间细胞在黏液表皮样癌组织发生中的可能作用相关。
