• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Nrf2 通过调节 PI3K/Akt 信号通路负调控黑色素生成。

Nrf2 negatively regulates melanogenesis by modulating PI3K/Akt signaling.

机构信息

Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, Korea.

Department of Anatomy, School of Medicine, Chungnam National University, Daejeon, Korea.

出版信息

PLoS One. 2014 Apr 24;9(4):e96035. doi: 10.1371/journal.pone.0096035. eCollection 2014.

DOI:10.1371/journal.pone.0096035
PMID:24763530
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3999113/
Abstract

Nrf2 plays a role in protection of cells against oxidative stress and xenobiotic damage by regulating cytoprotective genes. In this study, we investigated the effect of Nrf2 on melanogenesis in normal human melanocytes (NHMCs). When NHMCs were transduced with a recombinant adenovirus expressing Nrf2, melanin synthesis was significantly decreased. Consistent with this result, overexpression of Nrf2 decreased the expression of tyrosinase and tyrosinase-related protein 1. The inhibitory effect of Nrf2 was reversed by overexpression of Keap1, an intracellular regulator of Nrf2. Interestingly, Nrf2 overexpression resulted in marked activation of PI3K/Akt signaling. Conversely, inhibition of PI3K activity by treatment with wortmannin reversed the depigmentary effects of Nrf2. Taken together, these results strongly suggest that Nrf2 negatively regulates melanogenesis by modulating the PI3K/Akt signaling pathway.

摘要

Nrf2 通过调节细胞保护基因在细胞对抗氧化应激和外源性损伤中发挥作用。在这项研究中，我们研究了 Nrf2 对正常人黑素细胞（NHMC）中黑色素生成的影响。当 NHMC 被表达 Nrf2 的重组腺病毒转导时，黑色素合成显著减少。与这一结果一致，Nrf2 的过表达降低了酪氨酸酶和酪氨酸酶相关蛋白 1 的表达。Nrf2 的抑制作用被 Nrf2 的细胞内调节剂 Keap1 的过表达所逆转。有趣的是，Nrf2 的过表达导致 PI3K/Akt 信号通路的显著激活。相反，用渥曼青霉素处理抑制 PI3K 活性可逆转 Nrf2 的退色作用。总之，这些结果强烈表明，Nrf2 通过调节 PI3K/Akt 信号通路负调控黑色素生成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07c6/3999113/e680a602d808/pone.0096035.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07c6/3999113/11d2b131103d/pone.0096035.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07c6/3999113/02f4fa39930a/pone.0096035.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07c6/3999113/7bbb8bca353b/pone.0096035.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07c6/3999113/e680a602d808/pone.0096035.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07c6/3999113/11d2b131103d/pone.0096035.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07c6/3999113/02f4fa39930a/pone.0096035.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07c6/3999113/7bbb8bca353b/pone.0096035.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/07c6/3999113/e680a602d808/pone.0096035.g004.jpg

相似文献

1
Nrf2 negatively regulates melanogenesis by modulating PI3K/Akt signaling.Nrf2 通过调节 PI3K/Akt 信号通路负调控黑色素生成。
PLoS One. 2014 Apr 24;9(4):e96035. doi: 10.1371/journal.pone.0096035. eCollection 2014.
2
Curcumin inhibits melanogenesis in human melanocytes.姜黄素抑制人黑素细胞中的黑色素生成。
Phytother Res. 2012 Feb;26(2):174-9. doi: 10.1002/ptr.3517. Epub 2011 May 17.
3
Inhibition of melanogenesis by gallic acid: possible involvement of the PI3K/Akt, MEK/ERK and Wnt/β-catenin signaling pathways in B16F10 cells.没食子酸对黑色素生成的抑制作用：可能涉及 B16F10 细胞中的 PI3K/Akt、MEK/ERK 和 Wnt/β-catenin 信号通路。
Int J Mol Sci. 2013 Oct 14;14(10):20443-58. doi: 10.3390/ijms141020443.
4
Panaxatriol saponins attenuated oxygen-glucose deprivation injury in PC12 cells via activation of PI3K/Akt and Nrf2 signaling pathway.人参三醇皂苷通过激活PI3K/Akt和Nrf2信号通路减轻PC12细胞的氧糖剥夺损伤。
Oxid Med Cell Longev. 2014;2014:978034. doi: 10.1155/2014/978034. Epub 2014 May 11.
5
Baicalein protects human vitiligo melanocytes from oxidative stress through activation of NF-E2-related factor2 (Nrf2) signaling pathway.黄芩素通过激活 NF-E2 相关因子 2（Nrf2）信号通路保护人白癜风黑素细胞免受氧化应激。
Free Radic Biol Med. 2018 Dec;129:492-503. doi: 10.1016/j.freeradbiomed.2018.10.421. Epub 2018 Oct 18.
6
The antimycotic agent clotrimazole inhibits melanogenesis by accelerating ERK and PI3K-/Akt-mediated tyrosinase degradation.抗真菌剂克霉唑通过加速ERK和PI3K-/Akt介导的酪氨酸酶降解来抑制黑色素生成。
Exp Dermatol. 2015 May;24(5):386-8. doi: 10.1111/exd.12669. Epub 2015 Mar 9.
7
Essential roles of the PI3 kinase/Akt pathway in regulating Nrf2-dependent antioxidant functions in the RPE.PI3激酶/Akt信号通路在调节视网膜色素上皮细胞中Nrf2依赖的抗氧化功能方面的重要作用。
Invest Ophthalmol Vis Sci. 2008 Apr;49(4):1671-8. doi: 10.1167/iovs.07-1099.
8
Gomisin N Inhibits Melanogenesis through Regulating the PI3K/Akt and MAPK/ERK Signaling Pathways in Melanocytes.戈米辛N通过调节黑素细胞中的PI3K/Akt和MAPK/ERK信号通路来抑制黑色素生成。
Int J Mol Sci. 2017 Feb 22;18(2):471. doi: 10.3390/ijms18020471.
9
Partially purified components of Nardostachys chinensis suppress melanin synthesis through ERK and Akt signaling pathway with cAMP down-regulation in B16F10 cells.藏菖蒲的部分纯化成分通过下调 cAMP 抑制 ERK 和 Akt 信号通路来抑制 B16F10 细胞中的黑色素合成。
J Ethnopharmacol. 2011 Oct 11;137(3):1207-14. doi: 10.1016/j.jep.2011.07.047. Epub 2011 Jul 26.
10
NRF2-dependent glutamate-L-cysteine ligase catalytic subunit expression mediates insulin protection against hyperglycemia- induced brain endothelial cell apoptosis.NRF2 依赖性谷氨酸 - L - 半胱氨酸连接酶催化亚基的表达介导胰岛素对高血糖诱导的脑内皮细胞凋亡的保护作用。
Curr Neurovasc Res. 2006 Nov;3(4):249-61. doi: 10.2174/156720206778792876.

引用本文的文献

1
The Pathogenesis of Oxidative Stress-Induced Chloasma and Its Therapeutic Implications.氧化应激诱导黄褐斑的发病机制及其治疗意义。
Clin Cosmet Investig Dermatol. 2025 Apr 16;18:929-947. doi: 10.2147/CCID.S520625. eCollection 2025.
2
Integrated analysis of tyrosine-induced MiRNA and mRNA expression profiles in melanocytes reveals the regulatory role of miR-1560-3p in melanin deposition in Xichuan black-bone chickens.酪氨酸诱导的黑素细胞中 miRNA 和 mRNA 表达谱的综合分析揭示了 miR-1560-3p 在淅川乌骨鸡黑色素沉积中的调控作用。
BMC Genomics. 2025 Apr 7;26(1):348. doi: 10.1186/s12864-025-11543-8.
3
ROS and calcium signaling are critical determinant of skin pigmentation.

本文引用的文献

1
Roles of TLR7 in activation of NF-κB signaling of keratinocytes by imiquimod.TLR7 在咪喹莫特激活角质形成细胞 NF-κB 信号通路中的作用。
PLoS One. 2013 Oct 11;8(10):e77159. doi: 10.1371/journal.pone.0077159. eCollection 2013.
2
Comparison of Gene Expression Profiles between Keratinocytes, Melanocytes and Fibroblasts.角质形成细胞、黑素细胞和成纤维细胞之间基因表达谱的比较。
Ann Dermatol. 2013 Feb;25(1):36-45. doi: 10.5021/ad.2013.25.1.36. Epub 2013 Feb 14.
3
Loss of transcription factor nuclear factor-erythroid 2 (NF-E2) p45-related factor-2 (Nrf2) leads to dysregulation of immune functions, redox homeostasis, and intracellular signaling in dendritic cells.
活性氧（ROS）和钙信号传导是皮肤色素沉着的关键决定因素。
Cell Calcium. 2025 Jan;125:102987. doi: 10.1016/j.ceca.2024.102987. Epub 2024 Dec 20.
4
Joint Genomic and Transcriptomic Analysis Reveals Candidate Genes Associated with Plumage Color Traits in Matahu Ducks.联合基因组和转录组分析揭示了与麻鸭羽色性状相关的候选基因。
Animals (Basel). 2024 Oct 29;14(21):3111. doi: 10.3390/ani14213111.
5
A novel liquid plasma derivative inhibits melanogenesis through upregulation of Nrf2.一种新型液态等离子体衍生物通过上调 Nrf2 抑制黑色素生成。
Sci Rep. 2024 Sep 19;14(1):21851. doi: 10.1038/s41598-024-72750-z.
6
Unlocking the Bioactive Potential and Exploring Novel Applications for Portuguese Endemic .挖掘葡萄牙本土生物的生物活性潜力并探索其新应用。
Plants (Basel). 2024 Jul 15;13(14):1943. doi: 10.3390/plants13141943.
7
Immunoregulation: the interplay between metabolism and redox homeostasis.免疫调节：代谢与氧化还原稳态之间的相互作用。
Front Transplant. 2023 Nov 27;2:1283275. doi: 10.3389/frtra.2023.1283275. eCollection 2023.
8
Exploring the Influence of Cold Plasma on Epidermal Melanogenesis In Situ and In Vitro.探索冷等离子体对表皮黑色素生成的体内和体外影响。
Int J Mol Sci. 2024 May 10;25(10):5186. doi: 10.3390/ijms25105186.
9
Thiol starvation triggers melanoma state switching in an ATF4 and NRF2-dependent manner.硫醇饥饿以 ATF4 和 NRF2 依赖的方式触发黑色素瘤状态转换。
Redox Biol. 2024 Apr;70:103011. doi: 10.1016/j.redox.2023.103011. Epub 2023 Dec 27.
10
The Role of Oxidative Stress in Vitiligo: An Update on Its Pathogenesis and Therapeutic Implications.氧化应激在白癜风中的作用：发病机制及治疗意义的最新研究进展。
Cells. 2023 Mar 19;12(6):936. doi: 10.3390/cells12060936.
转录因子核因子红细胞 2（NF-E2）相关因子 2（Nrf2）的缺失导致树突状细胞免疫功能、氧化还原稳态和细胞内信号转导的失调。
J Biol Chem. 2012 Mar 23;287(13):10556-10564. doi: 10.1074/jbc.M111.322420. Epub 2012 Feb 6.
4
Acceleration of UVB-induced photoageing in nrf2 gene-deficient mice.Nrf2 基因缺陷型小鼠中 UVB 诱导的光老化加速。
Exp Dermatol. 2011 Aug;20(8):664-8. doi: 10.1111/j.1600-0625.2011.01292.x. Epub 2011 May 16.
5
Central role for cAMP signaling in pigmentation and UV resistance.环磷酸腺苷（cAMP）信号在色素沉着和抗紫外线方面起核心作用。
Cell Cycle. 2011 Jan 1;10(1):8-9. doi: 10.4161/cc.10.1.14292.
6
Transcriptional upregulation of Nrf2-dependent phase II detoxification genes in the involved epidermis of vitiligo vulgaris.寻常型白癜风皮损表皮 Nrf2 依赖性Ⅱ相解毒基因转录上调。
J Invest Dermatol. 2010 Dec;130(12):2781-9. doi: 10.1038/jid.2010.201. Epub 2010 Jul 22.
7
Curcumin suppresses alpha-melanocyte stimulating hormone-stimulated melanogenesis in B16F10 cells.姜黄素抑制 B16F10 细胞中 α-黑色素细胞刺激素刺激的黑色素生成。
Int J Mol Med. 2010 Jul;26(1):101-6.
8
Signaling pathways in melanosome biogenesis and pathology.黑色素体生物发生和病理学中的信号通路。
Int J Biochem Cell Biol. 2010 Jul;42(7):1094-104. doi: 10.1016/j.biocel.2010.03.023. Epub 2010 Apr 8.
9
Sulforaphane inhibited melanin synthesis by regulating tyrosinase gene expression in B16 mouse melanoma cells.萝卜硫素通过调节B16小鼠黑色素瘤细胞中酪氨酸酶基因的表达来抑制黑色素的合成。
Biosci Biotechnol Biochem. 2010;74(3):579-82. doi: 10.1271/bbb.90778. Epub 2010 Mar 7.
10
p38 regulates pigmentation via proteasomal degradation of tyrosinase.p38 通过蛋白酶体降解酪氨酸酶来调节色素沉着。
J Biol Chem. 2010 Mar 5;285(10):7288-99. doi: 10.1074/jbc.M109.070573. Epub 2010 Jan 6.