Alam R, Forsythe P A, Lett-Brown M A, Grant J A
Department of Medicine, University of Texas Medical Branch, Galveston 77550.
J Immunol. 1989 Oct 1;143(7):2280-4.
We have recently described a specific antagonist of histamine-releasing factors that inhibits histamine release from basophils and mast cells. This histamine release inhibitory factor (HRIF) is produced by PBMC upon stimulation with histamine as well as mitogens such as Con A. The objective of this study was to investigate the cellular origin of HRIF produced by PBMC. Monocytes, T cells, and B cells were isolated to 96 to 99% purity by a combination of plastic adherence, E rosetting, and negative selection with mAb (OKM1, OKT11, OKB7, OKT4, and OKT8) and C. Purified subpopulations were cultured with histamine or Con A and then the processed supernatants were assayed for the inhibition of HRF-induced histamine release from basophils. The results of this study suggest that the highest amount of HRIF is synthesized by B cells followed by T cells and monocytes. The B cell origin of HRIF was confirmed by abolishing the activity after incubation of the cells with OKB7 mAb and C. Both CD4- and CD8- T cells are capable of producing HRIF. In mixing experiments, the synthesis of HRIF by two different subpopulations has been less than additive. T + B cells produced most of the HRIF activity. Monocytes tended to suppress the synthesis of HRIF by B cells. The synthesis of HRIF by so many cell types suggests that a fine balance between HRIF and HRF may regulate the mediator release from basophils and mast cells.
我们最近描述了一种组胺释放因子的特异性拮抗剂，它能抑制嗜碱性粒细胞和肥大细胞释放组胺。这种组胺释放抑制因子（HRIF）是外周血单核细胞（PBMC）在组胺以及诸如刀豆蛋白A等丝裂原刺激下产生的。本研究的目的是调查PBMC产生的HRIF的细胞来源。通过塑料黏附、E花环形成以及用单克隆抗体（OKM1、OKT11、OKB7、OKT4和OKT8）和补体进行阴性选择相结合的方法，将单核细胞、T细胞和B细胞分离至纯度达96%至99%。将纯化的亚群用组胺或刀豆蛋白A培养，然后检测处理后的上清液对HRF诱导的嗜碱性粒细胞组胺释放的抑制作用。本研究结果表明，合成HRIF量最高的是B细胞，其次是T细胞和单核细胞。在用OKB7单克隆抗体和补体孵育细胞后活性消失，从而证实了HRIF的B细胞来源。CD4+和CD8+ T细胞都能够产生HRIF。在混合实验中，两个不同亚群合成的HRIF少于相加效应。T + B细胞产生了大部分的HRIF活性。单核细胞倾向于抑制B细胞合成HRIF。如此多细胞类型都能合成HRIF，这表明HRIF和HRF之间的精细平衡可能调节嗜碱性粒细胞和肥大细胞的介质释放。
