Alam R, Lewis D M, Olenchock S A
Immunology Section, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505.
Cell Immunol. 1988 Sep;115(2):447-59. doi: 10.1016/0008-8749(88)90197-9.
Guinea pig spleen cells, thymocytes, and blood mononuclear cells have been shown to produce a histamine releasing factor (HRF) spontaneously or after stimulation with PHA or specific antigens. In this study we have shown that antigenic stimulation of spleen cells obtained from animals immunized with high doses of antigen suppresses the spontaneous HRF production. Likewise, stimulation with Con A of spleen cells also inhibits the spontaneous HRF production. When the supernatants from Con A- and antigen-stimulated cultures were added to fresh thymocyte cultures, a significant inhibition of HRF production was observed. These supernatants also inhibited HRF-induced histamine release from mast cells. We have identified an inhibitor of HRF synthesis (IHS) and also a histamine release inhibitory factor (HRIF) by gel chromatography. Virtually all mitogen- and antigen-stimulated culture supernatants elaborated the activities of HRF, IHS, and HRIF in various quantities depending on the dose of antigen and the kind of adjuvant used for immunization. IHS has a MW of 22K-35K and 10K. This cytokine also inhibits DNA synthesis by thymocytes. HRIF has a MW of 15K-20K and 10K. It inhibits both HRF- and antigen-induced histamine release from lung mast cells. These results suggest that lymphocytes produce a variety of factors which function to regulate histamine release from mast cells.
豚鼠的脾细胞、胸腺细胞和血液单核细胞已被证明可自发产生组胺释放因子（HRF），或在受到PHA或特定抗原刺激后产生该因子。在本研究中，我们发现，用高剂量抗原免疫动物所获得的脾细胞经抗原刺激后，会抑制HRF的自发产生。同样，用刀豆蛋白A刺激脾细胞也会抑制HRF的自发产生。当将刀豆蛋白A和抗原刺激培养物的上清液添加到新鲜的胸腺细胞培养物中时，可观察到HRF产生受到显著抑制。这些上清液还抑制了HRF诱导的肥大细胞组胺释放。我们通过凝胶色谱法鉴定出了一种HRF合成抑制剂（IHS）以及一种组胺释放抑制因子（HRIF）。实际上，所有有丝分裂原和抗原刺激的培养上清液都会根据抗原剂量和用于免疫的佐剂种类，以不同数量呈现出HRF、IHS和HRIF的活性。IHS的分子量为22K - 35K和10K。这种细胞因子还会抑制胸腺细胞的DNA合成。HRIF的分子量为15K - 20K和10K。它可抑制HRF和抗原诱导的肺肥大细胞组胺释放。这些结果表明，淋巴细胞会产生多种因子，这些因子起到调节肥大细胞组胺释放的作用。
