Kim Ji Won, Woo Kyungbae, Kim Jeong Mi, Choi Mi Eun, Kim Young-Mo, Yang Su-Geun, Shim Bong Sup, Choi Jeong-Seok
Department of Otorhinolaryngology, Inha University, College of Medicine, 27 Inhang-ro, Jung-gu, Incheon 22332, Republic of Korea.
Department of Chemical Engineering, Inha University, 100 Inharo, Michuholgu, Incheon 22212, Republic of Korea.
Regen Biomater. 2020 Feb;7(1):47-52. doi: 10.1093/rb/rbz054. Epub 2020 Mar 3.
Nanocellulose has emerged for a wide range of applications in biomedical engineering because of its water absorption capacity, appropriate elasticity. We investigated the hemostatic and regenerative abilities of an expanding polyvinyl alcohol (PVA)-nanocellulose sponge on nasal mucosal defects. A 3 mm-diameter nasal defect was made in experimental rabbits. Rabbits were divided into four groups with control, vaseline, PVA and PVA-nanocellulose packing groups. After the defect was created, bleeding times and amounts were monitored. Packing materials were removed on experimental day (ED) 2. On ED 3, 7 and 14, histological analysis and immunohistochemical study for neutrophils were performed. Inflammatory cells were counted and epithelial thicknesses were evaluated. Bleeding amounts and times in the vaseline packing group were smaller than in the PVA groups. PVA-nanocellulose group showed less neutrophils than in the other groups on ED 7. Average epithelium thickness in the PVA-nanocellulose group was significantly smaller than in the control group at ED 7, but at ED 14, there was no significant intergroup difference. PVA-nanocellulose group had a significant lower inflammatory cell count than the control group on ED 7. PVA-nanocellulose sponge applied to nasal mucosal defects can significantly enhance mucosal regeneration during early wound healing.
由于具有吸水能力和适当的弹性，纳米纤维素在生物医学工程领域出现了广泛的应用。我们研究了一种膨胀型聚乙烯醇（PVA）-纳米纤维素海绵对鼻黏膜缺损的止血和再生能力。在实验兔身上制造了直径3毫米的鼻缺损。将兔子分为四组，分别为对照组、凡士林组、PVA组和PVA-纳米纤维素填充组。制造缺损后，监测出血时间和出血量。在实验第2天取出填充材料。在实验第3天、第7天和第14天，进行组织学分析和中性粒细胞的免疫组织化学研究。对炎症细胞进行计数并评估上皮厚度。凡士林填充组的出血量和出血时间比PVA组小。在实验第7天，PVA-纳米纤维素组的中性粒细胞比其他组少。在实验第7天，PVA-纳米纤维素组的平均上皮厚度明显小于对照组，但在实验第14天，组间无显著差异。在实验第7天，PVA-纳米纤维素组的炎症细胞计数明显低于对照组。应用于鼻黏膜缺损的PVA-纳米纤维素海绵可在伤口愈合早期显著促进黏膜再生。
