肠易激综合征患者循环 miR-150 和 miR-342-3p 水平升高。
Elevated circulating miR-150 and miR-342-3p in patients with irritable bowel syndrome.
机构信息
Digestive Disorders Unit, National Institute of Nursing Research, National Institutes of Health, DHHS, Bethesda, MD 20892, United States.
Digestive Disorders Unit, National Institute of Nursing Research, National Institutes of Health, DHHS, Bethesda, MD 20892, United States; Howard Hughes Medical Institute National Institutes of Health Research Scholar, Chevy Chase, MD 20815, United States.
出版信息
Exp Mol Pathol. 2014 Jun;96(3):422-5. doi: 10.1016/j.yexmp.2014.04.009. Epub 2014 Apr 21.
BACKGROUND AND AIMS
MicroRNAs (miRNAs) are small non-coding RNAs, which regulate gene expression and are thus of interest as diagnostic markers, and as clues to etiology and targets of intervention. This pilot study examined whether circulating miRNAs are differentially expressed in patients with IBS.
METHODS
miRNA microarrays (NanoString) were run on the whole blood of 43 participants.
RESULTS
hsa-miR-150 and hsa-miR-342-3p were found to be significantly elevated (FDR adjusted p≤0.05, ≥1.6 fold change) in IBS patients compared to healthy controls. Neither of these miRNAs showed any relationship to race or sex. hsa-miR-150 is associated with inflammatory bowel disorders and pain, and interacts with a protein kinase (AKT2) through which it may affect inflammatory pathways. hsa-miR-342-3p is predicted to interact with mRNAs involved in pain signaling, colonic motility, and smooth muscle function.
CONCLUSIONS
This preliminary study reports the association of two miRNAs, detected in whole blood, with IBS. These miRNAs link to pain and inflammatory pathways both of which are thought to be dysregulated in IBS. Larger sample sizes are needed to confirm their importance and potential as biomarkers.
背景和目的
微小 RNA（miRNA）是一种小的非编码 RNA，可调节基因表达，因此作为诊断标志物、病因线索和干预靶点而受到关注。本初步研究旨在探讨循环 miRNA 是否在 IBS 患者中存在差异表达。
方法
对 43 名参与者的全血进行 miRNA 微阵列（NanoString）检测。
结果
与健康对照组相比，IBS 患者的 hsa-miR-150 和 hsa-miR-342-3p 显著升高（FDR 调整的 p 值≤0.05，倍数变化≥1.6）。这两种 miRNA 均与种族或性别无关。hsa-miR-150 与炎症性肠病和疼痛有关，并且通过与蛋白激酶（AKT2）相互作用，可能影响炎症途径。hsa-miR-342-3p 被预测与涉及疼痛信号传导、结肠运动和平滑肌功能的 mRNA 相互作用。
结论
本初步研究报告了两种 miRNA 的关联，这些 miRNA 在全血中被检测到，与 IBS 相关。这些 miRNA 与疼痛和炎症途径相关，而这些途径在 IBS 中被认为失调。需要更大的样本量来确认它们作为生物标志物的重要性和潜力。
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