吸烟和无烟烟草使用者癌症风险的遗传毒性和分子机制评估。
Assessment of genotoxic and molecular mechanisms of cancer risk in smoking and smokeless tobacco users.
作者信息
Chandirasekar R, Kumar B Lakshman, Sasikala K, Jayakumar R, Suresh K, Venkatesan R, Jacob Raichel, Krishnapriya E K, Kavitha H, Ganesh G Karthik
机构信息
Unit of Human Genetics, Department of Zoology, Bharathiar University, Coimbatore 641 046, Tamilnadu, India.
Departments of Biotechnology and Zoology, Kongunadu Arts and Science College, Coimbatore 641 029, India.
出版信息
Mutat Res Genet Toxicol Environ Mutagen. 2014 Jun;767:21-7. doi: 10.1016/j.mrgentox.2014.04.007. Epub 2014 Apr 21.
Inexpensive forms of tobacco are widely used in developing countries such as India. We have evaluated genotoxicity endpoints (chromosome aberrations, micronucleus frequency, comet assay) and polymorphisms of the XRCC1 and p53 genes among smokers and smokeless tobacco (SLT) users in rural Tamilnadu, South India. Cytogenetic, DNA damage and SNP analyses were performed on peripheral blood samples; micronucleus frequency was measured in peripheral blood and buccal mucosa exfoliated cells. Both categories of tobacco users had elevated levels of genotoxic damage. SNP analysis of tobacco users revealed that 17% carry the XRCC1 gln399gln genotype and 19% carry the p53 pro72pro genotype. Both genotypes are associated with increased risk of cancer.
廉价烟草制品在印度等发展中国家广泛使用。我们评估了印度南部泰米尔纳德邦农村地区吸烟者和无烟烟草使用者的遗传毒性指标（染色体畸变、微核频率、彗星试验）以及XRCC1和p53基因的多态性。对外周血样本进行了细胞遗传学、DNA损伤和单核苷酸多态性分析；在 peripheral blood 和颊黏膜脱落细胞中测量微核频率。两类烟草使用者的遗传毒性损伤水平均有所升高。对烟草使用者的单核苷酸多态性分析显示，17%的人携带XRCC1 gln399gln基因型，19%的人携带p53 pro72pro基因型。这两种基因型均与癌症风险增加相关。
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