Hosseini Samira, Ibrahim Fatimah, Djordjevic Ivan, Koole Leo H
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur, 50603, Malaysia.
Analyst. 2014 Jun 21;139(12):2933-43. doi: 10.1039/c3an01789c.
Biosensor chips for immune-based assay systems have been investigated for their application in early diagnostics. The development of such systems strongly depends on the effective protein immobilization on polymer substrates. In order to achieve this complex heterogeneous interaction the polymer surface must be functionalized with chemical groups that are reactive towards proteins in a way that surface functional groups (such as carboxyl, -COOH; amine, -NH2; and hydroxyl, -OH) chemically or physically anchor the proteins to the polymer platform. Since the proteins are very sensitive towards their environment and can easily lose their activity when brought in close proximity to the solid surface, effective surface functionalization and high level of control over surface chemistry present the most important steps in the fabrication of biosensors. This paper reviews recent developments in surface functionalization and preparation of polymethacrylates for protein immobilization. Due to their versatility and cost effectiveness, this particular group of plastic polymers is widely used both in research and in industry.
用于基于免疫分析系统的生物传感器芯片已被研究用于早期诊断。此类系统的发展在很大程度上取决于蛋白质在聚合物基质上的有效固定。为了实现这种复杂的异质相互作用，聚合物表面必须用对蛋白质具有反应性的化学基团进行功能化，使得表面官能团（如羧基，-COOH；胺基，-NH2；以及羟基，-OH）通过化学或物理方式将蛋白质锚定到聚合物平台上。由于蛋白质对其环境非常敏感，并且在靠近固体表面时很容易失去活性，有效的表面功能化以及对表面化学的高度控制是生物传感器制造中最重要的步骤。本文综述了用于蛋白质固定的聚甲基丙烯酸酯表面功能化和制备方面的最新进展。由于其多功能性和成本效益，这类特殊的塑料聚合物在研究和工业中都得到了广泛应用。
