从一家三级医院的尿路感染患者中分离出的大肠杆菌及其药敏分析。
Isolation and antibiotic susceptibility of E. coli from urinary tract infections in a tertiary care hospital.
机构信息
Sumera Sabir, B.S (Microbiology), Department of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Aftab Ahmad Anjum, PhD (Microbiology), Department of Microbiology, Faculty of Veterinary Science, University of Veterinary and Animal Sciences, Lahore, Pakistan.
出版信息
Pak J Med Sci. 2014 Mar;30(2):389-92.
OBJECTIVE
The study was conducted to isolate and determine the antibiotic resistance in E. coli from urinary tract infections in a tertiary care hospital, Lahore.
METHODS
Urine samples (n=500) were collected from patients with signs and symptoms of Urinary tract infections. Bacteria were isolated and identified by conventional biochemical profile. Antibiotic resistance pattern of E. coli against different antibiotic was determined by Kirby-Baur method.
RESULTS
Bacterial etiological agent was isolated from 402 samples with highest prevalence of E. coli (321, 80%) followed by Staphylococcus aureus (9.4%), Proteus species (5.4%) and Pseudomonas species (5.2%). The E. coli were highly resistant to penicillin (100%), amoxicillin (100%) and cefotaxime (89.7%), followed by intermediate level of resistance to ceftazidime (73.8%), cephradine (73.8%), tetracycline (69.4%), doxycycline (66.6%), augmentin (62.6%), gentamycin (59.8%), cefuroxime (58.2%), ciprofloxacin (54.2%), cefaclor (50%), aztreonam (44.8%), ceftriaxone (43.3%), imipenem (43.3%), and low level of resistance to streptomycin (30%), kanamycin (19.9%), tazocin (14%), amikacin (12.7%) and lowest to norfloxacin (11.2%). Out of 321 E. coli isolates, 261 (81%) were declared as multiple drug resistant and 5 (1.5%) were extensive drug resistant.
CONCLUSION
It is concluded that most of the urinary tract infections in human are caused by multiple drug resistant E. coli.
目的
本研究旨在从拉合尔一家三级护理医院的尿路感染患者中分离并确定大肠杆菌的抗生素耐药性。
方法
收集有尿路感染症状和体征的患者的尿液样本（n=500）。通过常规生化特征对细菌进行分离和鉴定。采用 Kirby-Baur 法测定大肠杆菌对不同抗生素的耐药模式。
结果
从 402 份样本中分离出细菌病原体，其中大肠杆菌（321 株，80%）的检出率最高，其次是金黄色葡萄球菌（9.4%）、变形杆菌属（5.4%）和铜绿假单胞菌属（5.2%）。大肠杆菌对青霉素（100%）、阿莫西林（100%）和头孢噻肟（89.7%）高度耐药，其次是头孢他啶（73.8%）、头孢唑林（73.8%）、四环素（69.4%）、强力霉素（66.6%）、复方新诺明（62.6%）、庆大霉素（59.8%）、头孢呋辛（58.2%）、环丙沙星（54.2%）、头孢克洛（50%）、氨曲南（44.8%）、头孢曲松（43.3%）、亚胺培南（43.3%）耐药程度中等，对链霉素（30%）、卡那霉素（19.9%）、他唑巴坦（14%）、阿米卡星（12.7%）耐药程度低，对诺氟沙星（11.2%）耐药程度最低。在 321 株大肠杆菌分离株中，261 株（81%）被判定为多重耐药，5 株（1.5%）为广泛耐药。
结论
综上所述，人类尿路感染主要由多重耐药大肠杆菌引起。
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