Sugiyama M, Minamoto N, Kinjo T, Hirayama N, Sasaki H, Yoshikawa Y, Yamanouchi K
Department of Veterinary Public Health, Faculty of Agriculture, Gifu University, Japan.
J Gen Virol. 1989 Oct;70 ( Pt 10):2605-13. doi: 10.1099/0022-1317-70-10-2605.
Twenty-four monoclonal antibodies (MAbs) against rinderpest virus (RPV) were established and characterized by several serological tests. Of the 24 MAbs. 10 recognized the nucleoprotein (NP), six the phosphoprotein (P), four the haemagglutinin (H), and two the fusion (F) protein as determined by radioimmunoprecipitation assay. The specificities of the remaining two MAbs could not be determined. From a competitive binding assay using MAbs against each structural protein, at least five, four and two separate antigenic sites were identified on the NP, P and H proteins, respectively. MAbs against the H protein neutralized the infectivity of the virus, but those against the F protein were only neutralizing in the presence of guinea-pig complement. The reactivities of each of the MAbs for other strains of morbillivirus were tested using an indirect immunofluorescent antibody assay. The MAbs against four out five antigenic sites on the NP showed cross-reactivity amongst all the strains of morbillivirus tested whereas the fifth antibody reacted only with RPV. Of the antibodies specific for the P protein, the antibody against one site was cross-reactive with all the strains of RPV, measles virus (MV) and canine distemper virus (CDV), the antibody against another site was reactive with RPV and MV but not with CDV, and the antibodies against the other two sites were specific for RPV.
制备了24种抗牛瘟病毒（RPV）的单克隆抗体（MAb），并通过多种血清学试验对其进行了表征。通过放射免疫沉淀试验确定，在这24种单克隆抗体中，10种识别核蛋白（NP），6种识别磷蛋白（P），4种识别血凝素（H），2种识别融合（F）蛋白。其余两种单克隆抗体的特异性无法确定。通过使用针对每种结构蛋白的单克隆抗体进行竞争结合试验，分别在NP、P和H蛋白上鉴定出至少5个、4个和2个独立的抗原位点。针对H蛋白的单克隆抗体可中和病毒的感染性，但针对F蛋白的单克隆抗体仅在存在豚鼠补体的情况下具有中和作用。使用间接免疫荧光抗体试验检测了每种单克隆抗体对其他麻疹病毒株的反应性。针对NP上五个抗原位点中的四个的单克隆抗体在所有测试的麻疹病毒株中均表现出交叉反应，而第五种抗体仅与牛瘟病毒反应。在针对P蛋白的特异性抗体中，针对一个位点的抗体与所有牛瘟病毒株、麻疹病毒（MV）和犬瘟热病毒（CDV）具有交叉反应，针对另一个位点的抗体与牛瘟病毒和麻疹病毒反应，但与犬瘟热病毒不反应，针对其他两个位点的抗体对牛瘟病毒具有特异性。
