Rajkumar Hemalatha, Mahmood Naseha, Kumar Manoj, Varikuti Sudarshan Reddy, Challa Hanumanth Reddy, Myakala Shiva Prakash
Department of Microbiology and Immunology, National Institute of Nutrition, ICMR, Hyderabad 500007, India.
Mediators Inflamm. 2014;2014:348959. doi: 10.1155/2014/348959. Epub 2014 Mar 26.
To evaluate the effects of probiotic (VSL#3) and omega-3 fatty acid on insulin sensitivity, blood lipids, and inflammation, we conducted a clinical trial in 60 overweight (BMI > 25), healthy adults, aged 40-60 years. After initial screening the subjects were randomized into four groups with 15 per group. The four groups received, respectively, placebo, omega-3 fatty acid, probiotic VSL#3, or both omega-3 and probiotic, for 6 weeks. Blood and fecal samples were collected at baseline and after 6 weeks. The probiotic (VSL#3) supplemented group had significant reduction in total cholesterol, triglyceride, LDL, and VLDL and had increased HDL (P < 0.05) value. VSL#3 improved insulin sensitivity (P < 0.01), decreased hsCRP, and favorably affected the composition of gut microbiota. Omega-3 had significant effect on insulin sensitivity and hsCRP but had no effect on gut microbiota. Addition of omega-3 fatty acid with VSL#3 had more pronounced effect on HDL, insulin sensitivity and hsCRP. Subjects with low HDL, insulin resistance, and high hsCRP had significantly lower total lactobacilli and bifidobacteria count and higher E. coli and bacteroides count.
为评估益生菌（VSL#3）和ω-3脂肪酸对胰岛素敏感性、血脂及炎症的影响，我们对60名年龄在40至60岁之间、超重（BMI>25）的健康成年人进行了一项临床试验。在初步筛选后，将受试者随机分为四组，每组15人。四组分别接受安慰剂、ω-3脂肪酸、益生菌VSL#3或ω-3脂肪酸与益生菌联合治疗，为期6周。在基线和6周后采集血液和粪便样本。补充益生菌（VSL#3）的组总胆固醇、甘油三酯、低密度脂蛋白和极低密度脂蛋白显著降低，高密度脂蛋白升高（P<0.05）。VSL#3改善了胰岛素敏感性（P<0.01），降低了高敏C反应蛋白，并对肠道微生物群的组成产生了有利影响。ω-3对胰岛素敏感性和高敏C反应蛋白有显著影响，但对肠道微生物群无影响。ω-3脂肪酸与VSL#3联合使用对高密度脂蛋白、胰岛素敏感性和高敏C反应蛋白有更显著的影响。高密度脂蛋白水平低、胰岛素抵抗和高敏C反应蛋白水平高的受试者总乳酸菌和双歧杆菌计数显著较低，大肠杆菌和拟杆菌计数较高。
