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两个新的缺氧诱导乙烯反应因子，它们与柿子脱涩相关基因的启动子相互作用。

Two novel anoxia-induced ethylene response factors that interact with promoters of deastringency-related genes from persimmon.

作者信息

Min Ting, Fang Fang, Ge Hang, Shi Yan-na, Luo Zheng-rong, Yao Yun-cong, Grierson Donald, Yin Xue-ren, Chen Kun-song

机构信息

Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou, PR China.

Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, PR China.

出版信息

PLoS One. 2014 May 7;9(5):e97043. doi: 10.1371/journal.pone.0097043. eCollection 2014.

DOI:10.1371/journal.pone.0097043
PMID:24805136
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4013125/
Abstract

A hypoxic environment is generally undesirable for most plants and stimulates anaerobic metabolism. It is a beneficial treatment, however, for the removal of astringency from persimmon to improve the fruit quality after harvest. High soluble tannins (SCTs) content is one of most important causes of astringency. High CO2 (95%) treatment effectively reduced SCTs in both "Mopan" and "Gongcheng-shuishi" persimmon fruit by causing increases in acetaldehyde. Using RNA-seq and realtime PCR, twelve ethylene response factor genes (DkERF11-22) were isolated and characterized, to determine those responsive to high CO2 treatment. Only two genes, DkERF19 and DkERF22, showed trans-activation effects on the promoters of deastringency-related genes pyruvate decarboxylase genes (DkPDC2 and DkPDC3) and the transcript levels of these genes was enhanced by hypoxia. Moreover, DkERF19 and the previously isolated DkERF9 had additive effects on activating the DkPDC2 promoter. Taken together, these results provide further evidence that transcriptome changes in the level of DkERF mRNAs regulate deastringency-related genes and their role in the mechanism of persimmon fruit deastringency is discussed.

摘要

缺氧环境通常对大多数植物不利，并会刺激无氧代谢。然而，对于去除柿子的涩味以提高采后果实品质而言，这是一种有益的处理方法。高可溶性单宁（SCTs）含量是涩味的最重要原因之一。高二氧化碳（95%）处理通过导致乙醛增加，有效降低了“磨盘”和“恭城水柿”果实中的SCTs。利用RNA测序和实时定量PCR，分离并鉴定了12个乙烯响应因子基因（DkERF11 - 22），以确定那些对高二氧化碳处理有响应的基因。只有两个基因，DkERF19和DkERF22，对脱涩相关基因丙酮酸脱羧酶基因（DkPDC2和DkPDC3）的启动子表现出反式激活作用，并且这些基因的转录水平在缺氧条件下会增强。此外，DkERF19和先前分离的DkERF9对激活DkPDC2启动子具有累加效应。综上所述，这些结果提供了进一步的证据，表明DkERF mRNA水平的转录组变化调节脱涩相关基因，并讨论了它们在柿子果实脱涩机制中的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6105/4013125/835befb5df50/pone.0097043.g008.jpg
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3
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9
Expression of ethylene response genes during persimmon fruit astringency removal.在柿子果实脱涩过程中乙烯应答基因的表达。
Planta. 2012 May;235(5):895-906. doi: 10.1007/s00425-011-1553-2. Epub 2011 Nov 20.
10
Oxygen sensing in plants is mediated by an N-end rule pathway for protein destabilization.植物中的氧感应是通过一种 N 端规则途径介导的蛋白质不稳定性途径来实现的。
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