He Jincan, Huang Meiying, Wang Dongmei, Zhang Zhuomin, Li Gongke
School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
J Pharm Biomed Anal. 2014 Dec;101:84-101. doi: 10.1016/j.jpba.2014.04.017. Epub 2014 Apr 24.
Sample preparation is a fundamental and essential step in almost all the analytical procedures, especially for the analysis of complex samples like biological and environmental samples. In past decades, with advantages of superparamagnetic property, good biocompatibility and high binding capacity, functionalized magnetic materials have been widely applied in various processes of sample preparation for biological analysis. In this paper, the recent advancements of magnetic separation techniques based on magnetic materials in the field of sample preparation for biological analysis were reviewed. The strategy of magnetic separation techniques was summarized. The synthesis, stabilization and bio-functionalization of magnetic nanoparticles were reviewed in detail. Characterization of magnetic materials was also summarized. Moreover, the applications of magnetic separation techniques for the enrichment of protein, nucleic acid, cell, bioactive compound and immobilization of enzyme were described. Finally, the existed problems and possible trends of magnetic separation techniques for biological analysis in the future were proposed.
样品制备几乎是所有分析程序中的一个基本且关键的步骤，尤其是对于诸如生物和环境样品等复杂样品的分析。在过去几十年中，功能化磁性材料凭借其超顺磁性、良好的生物相容性和高结合能力等优点，已广泛应用于生物分析样品制备的各个过程。本文综述了基于磁性材料的磁分离技术在生物分析样品制备领域的最新进展。总结了磁分离技术的策略。详细综述了磁性纳米颗粒的合成、稳定化及生物功能化。还总结了磁性材料的表征。此外，描述了磁分离技术在蛋白质、核酸、细胞、生物活性化合物富集及酶固定化方面的应用。最后，提出了生物分析磁分离技术目前存在的问题及未来可能的发展趋势。
