Rotti Harish, Guruprasad Kanive Parashiva, Nayak Jayakrishna, Kabekkodu Shama Prasada, Kukreja Harpreet, Mallya Sandeep, Nayak Jyothi, Bhradwaj Ramachandra C, Gangadharan G G, Prasanna B V, Raval Ritu, Kamath Asha, Gopinath Puthiya Mundyat, Kondaiah Paturu, Satyamoorthy Kapaettu
Division of Biotechnology, School of Life Sciences, Manipal University, Manipal, Karnataka, India.
Department of Shalyatantra/ Roganidana, Shri Dharmasthala Manjunatheshwara College of Ayurveda, Udupi, Karnataka, India.
J Ayurveda Integr Med. 2014 Jan;5(1):43-9. doi: 10.4103/0975-9476.128857.
Human variations related to immune response and disease susceptibility is well-documented in Ayurveda. Prakriti (body constitution) is the basic constitution of an individual established at the time of birth and distinguishes variations, into three broad phenotype categories such as vata, pitta and kapha. Variation in immune response is often attributed to and measured from the difference in cluster differentiation (CD) markers expressed in lymphocytes. Currently, there are no reports available on the expression of CD markers related to prakriti.
This is a pilot study performed to evaluate a panel of lymphocyte subset CD markers in dominant prakriti individuals.
Immunophenotyping was carried out using whole blood from a total of healthy 222 subjects, who are grouped into kapha (n = 95), pitta (n = 57) and vata (n = 70) prakritis. CD markers such as CD3, CD4, CD8, CD14, CD25, CD56, CD69, CD71 and HLA-DR were analyzed using flow cytometry method. Differences between groups were analyzed using one-way ANOVA or Kruskal-Wallis analysis of variance (ANOVA) and multiple comparisons between groups were performed by Bonferroni or Mann-Whitney U test with corrections for type I error respectively. Significance was evaluated by ANOVA and Pearson's correlation.
We observed a significant difference (P < 0.05) in the expression of CD markers such as CD14 (monocytes), CD25 (activated B cells) and CD56 (Natural killer cells) between different prakriti groups. CD25 and CD56 expression was significantly higher in kapha prakriti samples than other prakriti groups. Similarly, slightly higher levels of CD14 were observed in pitta prakriti samples.
Significant difference in the expression of CD14, CD25 and CD56 markers between three different prakriti is demonstrated. The increased level of CD25 and CD56 in kapha prakriti may indicate ability to elicit better immune response, which is in conformity with textual references in Ayurveda.
阿育吠陀医学中已有充分文献记载与免疫反应和疾病易感性相关的人类变异。体质是个体出生时确立的基本体质，可分为三种广泛的表型类别，即风型、火型和水型。免疫反应的差异通常归因于淋巴细胞中表达的分化群（CD）标志物的差异，并以此进行测量。目前，尚无关于与体质相关的CD标志物表达的报道。
这是一项初步研究，旨在评估占主导地位的体质个体中的一组淋巴细胞亚群CD标志物。
使用来自总共222名健康受试者的全血进行免疫表型分析，这些受试者被分为水型（n = 95）、火型（n = 57）和风型（n = 70）体质。使用流式细胞术方法分析CD3、CD4、CD8、CD14、CD25、CD56、CD69、CD71和HLA - DR等CD标志物。组间差异采用单因素方差分析或Kruskal - Wallis方差分析进行分析，组间多重比较分别采用Bonferroni或Mann - Whitney U检验并对I型错误进行校正。通过方差分析和Pearson相关性评估显著性。
我们观察到不同体质组之间CD标志物如CD14（单核细胞）、CD25（活化B细胞）和CD56（自然杀伤细胞）的表达存在显著差异（P < 0.05）。水型体质样本中CD25和CD56的表达显著高于其他体质组。同样，在火型体质样本中观察到CD14水平略高。
证明了三种不同体质之间CD14、CD25和CD56标志物表达存在显著差异。水型体质中CD25和CD56水平的升高可能表明其引发更好免疫反应的能力，这与阿育吠陀医学的文献记载相符。
