International Graduate School of Arts and Sciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama 236-0027, Japan.
Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237-0061, Japan.
Int J Syst Evol Microbiol. 2014 Aug;64(Pt 8):2712-2717. doi: 10.1099/ijs.0.062620-0. Epub 2014 May 20.
Polyvinyl-alcohol-degrading bacteria were isolated from the fruit of a grape in Yokosuka, Japan. The isolated strain, Zumi 37(T), was a Gram-stain-negative, rod-shaped, motile, non-spore-forming and strictly aerobic chemo-organotroph, showing optimal growth at pH 7.5, 30 °C and 0.1% (w/v) NaCl. The major respiratory quinone was Q-8. The predominant fatty acids were iso-C(15 : 0), C(16 : 0) and C(16 : 1)ω7c. The major polyamines were homospermidine and putrescine. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of the novel strain was 64.2 mol%. 16S rRNA gene sequence comparison revealed that strain Zumi 37(T) belongs to the family Sinobacteraceae within the class Gammaproteobacteria. Steroidobacter denitrificans DSM 18526(T) was the most closely related species with a validly published name, with 98.0% similarity based on 16S rRNA gene sequence comparison (and showed less than 87.5% sequence similarity to members of the genera Alkanibacter, Fontimonas, Hydrocarboniphaga, Nevskia and Solimonas with known 16S rRNA gene sequences). Phenotypes for growth under aerobic conditions and on complex media and major fatty acid composition, differed greatly from those of with comparatively high 16S rRNA gene sequence similarity. Based on phylogenetic, phenotypic and chemotaxonomic evidence, it is proposed that strain Zumi 37(T) represents a novel species in a new genus for which the name Povalibacter uvarum gen. nov., sp. nov. is proposed. The type strain of the type species is Zumi 37(T) ( = JCM 18749(T) = DSM 26723(T)).
从日本横须贺的一种葡萄果实中分离到聚乙烯醇降解细菌。分离到的菌株 Zumi 37(T)是革兰氏阴性、杆状、能动、非孢子形成且严格需氧的化能有机营养型细菌，在 pH7.5、30°C 和 0.1%（w/v）NaCl 下生长最佳。主要的呼吸醌是 Q-8。主要的脂肪酸是 iso-C(15 : 0)、C(16 : 0)和 C(16 : 1)ω7c。主要的多胺是腐胺和亚精胺。主要的极性脂是双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。新菌株的 DNA G+C 含量为 64.2mol%。16S rRNA 基因序列比较表明，菌株 Zumi 37(T)属于γ变形菌纲的 Sinobacteraceae 科。与已发表名称的最密切相关的种是 Steroidobacter denitrificans DSM 18526(T)，基于 16S rRNA 基因序列比较相似度为 98.0%（与具有已知 16S rRNA 基因序列的 Alkanibacter、Fontimonas、Hydrocarboniphaga、Nevskia 和 Solimonas 属成员的相似度小于 87.5%）。在有氧条件下和复杂培养基上生长的表型以及主要脂肪酸组成与具有较高 16S rRNA 基因序列相似度的菌株有很大不同。基于系统发育、表型和化学分类学证据，建议将菌株 Zumi 37(T)代表一个新属的新种，命名为 Povalibacter uvarum 属，种名新种。模式种为 Zumi 37(T)（=JCM 18749(T)=DSM 26723(T)）。
