Wang Xiaojing, Miao Qingqing, Song Tingjie, Yuan Qingpan, Gao Jinhao, Liang Gaolin
CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry & Collaborative Innovation Center of Suzhou Nano Science and Technology, University of Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui 230026, China.
Analyst. 2014 Jul 7;139(13):3360-4. doi: 10.1039/c4an00410h.
Herein, we report the development of a new fluorescent switch for sequential and selective sensing of Cu(2+) and L-histidine (L-His) in vitro and in living cells for the first time. In the absence of metal ions, Ac-SAACQ-Gly-Gly-Gly-Lys (FITC) (1) exhibits comparable fluorescence to that of free FITC. In the presence of metal ions, 1 selectively coordinates to Cu(2+) , causing its fluorescence emission to be quenched via photoinduced electron transfer. Interestingly, the as-formed 1–Cu(2+) complex selectively responds to L-His among the 20 natural amino acids by turning its fluorescence on. This property of fluorescence switch of 1 was successfully applied for qualitatively and quantitatively sensing Cu(2+) and L-His in vitro. Using this dual functional probe, we also sequentially imaged Cu(2+) and L-His in living HepG2 cells. Our new probe 1 could be applied for not only environmental monitoring but also biomolecule detection in the near future.
在此，我们首次报道了一种新型荧光开关的开发，该开关可在体外和活细胞中对铜离子（Cu(2+)）和L-组氨酸（L-His）进行顺序和选择性传感。在没有金属离子的情况下，Ac-SAACQ-甘氨酸-甘氨酸-甘氨酸-赖氨酸（异硫氰酸荧光素）（1）的荧光与游离异硫氰酸荧光素相当。在存在金属离子的情况下，1选择性地与Cu(2+)配位，通过光诱导电子转移使其荧光发射猝灭。有趣的是，形成的1-Cu(2+)配合物在20种天然氨基酸中对L-His有选择性响应，通过开启其荧光来实现。1的这种荧光开关特性成功地应用于体外定性和定量检测Cu(2+)和L-His。使用这种双功能探针，我们还对活的肝癌细胞系HepG2细胞中的Cu(2+)和L-His进行了顺序成像。我们的新型探针1在不久的将来不仅可用于环境监测，还可用于生物分子检测。
