Wang Nini, Xu Guobo, Fang Yang, Yang Tao, Zhao Hai, Li Guoyou
Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Molecules. 2014 May 22;19(5):6623-34. doi: 10.3390/molecules19056623.
Chemical investigation on the constituents of Landoltia punctata led to the isolation and identification of 17 compounds, four of which were new and identified as (3b,24S)-9,19-cycloartane-3,22,24,25-tetraol 3-O-[b-D-glucopyranosyl-(1→2)]-[b-D-glucopyranosyl-(1→6)]-b-D-glucopyranoside (1), (3b,24S)-9,19-cycloartane-3,24,25-triol 3-O-[b-d-glucopyranosyl-(1→2)]-[b-D-glucopyranosyl-(1→6)]-b-D-glucopyranoside (2), 3,4'-dihydroxy-7,3'-dimethoxyflavan-5-O-b-D-glucopyranoside (3) and 3,4'-dihydroxy-4,7,3'-trimethoxyflavan-5-O-b-D-glucopyranoside (4). Their structures were elucidated by spectroscopic, chemical, and biochemical methods. Thus, cycloartane triterpenoids were discovered in the Lemnaceae family for the first time. Compound 3 showed antioxidant capacity in the positively charged 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical (ABTS+•) and superoxide anion radical scavenging assays.
对浮叶眼子菜成分的化学研究导致分离并鉴定出17种化合物，其中4种为新化合物，分别鉴定为(3b,24S)-9,19-环阿尔廷烷-3,22,24,25-四醇3-O-[β-D-吡喃葡萄糖基-(1→2)]-[β-D-吡喃葡萄糖基-(1→6)]-β-D-吡喃葡萄糖苷(1)、(3b,24S)-9,19-环阿尔廷烷-3,24,25-三醇3-O-[β-D-吡喃葡萄糖基-(1→2)]-[β-D-吡喃葡萄糖基-(1→6)]-β-D-吡喃葡萄糖苷(2)、3,4'-二羟基-7,3'-二甲氧基黄烷-5-O-β-D-吡喃葡萄糖苷(3)和3,4'-二羟基-4,7,3'-三甲氧基黄烷-5-O-β-D-吡喃葡萄糖苷(4)。通过光谱、化学和生化方法阐明了它们的结构。因此，首次在浮萍科中发现了环阿尔廷烷三萜类化合物。化合物3在带正电荷的2,2'-偶氮双(3-乙基苯并噻唑啉-6-磺酸)自由基(ABTS+•)和超氧阴离子自由基清除试验中表现出抗氧化能力。
