Graduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan.
Graduate Institute of Electronics Engineering, National Taiwan University, Taipei, Taiwan.
Biosens Bioelectron. 2014 Nov 15;61:112-8. doi: 10.1016/j.bios.2014.05.010. Epub 2014 May 13.
For on-site clinical diagnosis of biomolecules, the detection performances of most point-of-care (POC) biosensor devices are limited by undesired cross-detection of other non-analyte proteins in patient serum samples and other complex samples. To conquer this obstacle, this work presents a fully integrated bottom-gate poly-silcion nanowire (polySi NW) biosensor system-on-chip (SoC) to enhance the detection performance of cardiac-specific troponin-I (cTnI) concentration levels in serum samples. By applying proper electrical potential at the bottom gate under polySi NW biosensor, the biosensor response to cTnI biomarker can be improved by at least 16 fold in 50% phantom serum samples. The experimental result shows its detection range is from 3.2 × 10(-13)M(mol l(-1)) to 3.2 × 10(-10)M. This enhancement can be attributed to the electrostatic interactions between target biomolecules and voltage-applied bottom gate electrodes. This is the first time that a polySi NW CMOS biosensor chip has shown feasibilities to detect specific biomarkers in serum samples. Therefore, the developed technology paves the way toward on-field applications of CMOS compatible SiNW biosensing technologies and it can be employed for future biomolecular analysis in on-site serum diagnosis applications.
对于生物分子的现场临床诊断，大多数即时检测 (POC) 生物传感器设备的检测性能受到患者血清样本和其他复杂样本中其他非分析物蛋白质的不希望的交叉检测的限制。为了克服这一障碍，本工作提出了一种完全集成的底栅多晶硅纳米线 (polySi NW) 生物传感器片上系统 (SoC)，以提高血清样本中心脏特异性肌钙蛋白 I (cTnI) 浓度水平的检测性能。通过在 polySi NW 生物传感器的底栅施加适当的电势，可以将生物传感器对 cTnI 生物标志物的响应至少提高 16 倍，在 50%的模拟血清样本中。实验结果表明其检测范围为 3.2×10(-13)M(mol l(-1))至 3.2×10(-10)M。这种增强可以归因于目标生物分子与施加电压的底栅电极之间的静电相互作用。这是首次证明 polySi NW CMOS 生物传感器芯片具有在血清样本中检测特定生物标志物的可行性。因此，所开发的技术为现场应用兼容 CMOS 的 SiNW 生物传感技术铺平了道路，并且可以用于现场血清诊断应用中的未来生物分子分析。
