Ghanizadeh Gerayeli Farhad, Hosseini Farnaz, Bagheri Zohreh, Savardashtaki Amir, Shabaninejad Zahra, Amani Ali Mohammad, Najafipour Sohrab
Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Int J Anal Chem. 2020 Jun 17;2020:5054864. doi: 10.1155/2020/5054864. eCollection 2020.
Zidovudine (ZDV) is an antiviral drug against HIV that was approved by the FDA on March, 1987. It is a reverse transcriptase inhibitor. This type of drug stops the reproduction of DNA and decreases the amount of the virus in the patients' blood. Due to the ability of forming various molecular bonds, silver nanoparticles (AgNPs) are widely used for the detection of large range of agents, including drugs. In this study, we synthesized AgNP-modified -cyclodextrin (-CD) using green synthesis for the sensitive and selective zidovudine (ZDV) determination. Characterization of nanoparticles was done using different methods including infrared (IR) spectroscopy, ultraviolet-visible (UV-Vis) spectroscopy, transmission electron microscopy (TEM), and also, X-ray diffraction (XRD) patterns. The AgNP -max peak was at approximately 405 nm. In the presence of ZDV, yellow solution was turned to red color, and surface plasmon absorption band was dramatically centered at 560 nm. ZDV was determined in the range of 50-500 M, and the detection limit value was obtained as 42 M. The sensor was used to determine ZDV in tablets with good recovery.
齐多夫定（ZDV）是一种抗HIV的抗病毒药物，于1987年3月获得美国食品药品监督管理局（FDA）批准。它是一种逆转录酶抑制剂。这类药物可阻止DNA的复制并减少患者血液中的病毒量。由于能够形成各种分子键，银纳米颗粒（AgNPs）被广泛用于检测包括药物在内的多种物质。在本研究中，我们采用绿色合成法合成了AgNP修饰的β-环糊精（β-CD），用于灵敏且选择性地测定齐多夫定（ZDV）。使用包括红外（IR）光谱、紫外-可见（UV-Vis）光谱、透射电子显微镜（TEM）以及X射线衍射（XRD）图谱等不同方法对纳米颗粒进行了表征。AgNP的最大吸收峰位于约405nm处。在存在ZDV的情况下，黄色溶液变为红色，表面等离子体吸收带显著集中在560nm处。ZDV的测定范围为50 - 500μM，检测限值为42μM。该传感器用于测定片剂中的ZDV，回收率良好。
