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蜂胶降低氧化型低密度脂蛋白刺激的人脐静脉内皮细胞中磷酯酰胆碱特异性磷脂酶 C 活性并增加膜联蛋白 A7 水平。

Propolis Reduces Phosphatidylcholine-Specific Phospholipase C Activity and Increases Annexin a7 Level in Oxidized-LDL-Stimulated Human Umbilical Vein Endothelial Cells.

机构信息

School of Life Science, Liaocheng University, Liaocheng 252059, China ; College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.

School of Life Science, Liaocheng University, Liaocheng 252059, China.

出版信息

Evid Based Complement Alternat Med. 2014;2014:465383. doi: 10.1155/2014/465383. Epub 2014 Apr 22.

DOI:10.1155/2014/465383
PMID:24864152
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4016937/
Abstract

To understand the mechanisms underlying the regulating dyslipidemia action of Chinese propolis and Brazilian green propolis, we investigated their effects on phosphatidylcholine-specific phospholipase C (PC-PLC) activity and annexin a7 (ANXA7) level which play crucial roles in the control of the progress of atherosclerosis. Furthermore, active oxygen species (ROS) levels, nuclear factor-KappaB p65 (NF- κ B p65), and mitochondrial membrane potential (MMP) were also investigated in oxidized-LDL- (ox-LDL-) stimulated human umbilical vein endothelial cells (HUVECs). Our data indicated that the treatment of both types of propolis 12.5  μ g/mL significantly increased cell viability and attenuated apoptosis rate, increased ANXA7 level, and decreased PC-PLC activity. Both types of propolis also inhibited ROS generation as well as the subsequent MMP collapse, and NF- κ B p65 activation induced by ox-LDL in HUVECs. Our results also indicated that Chinese propolis and Brazilian green propolis had similar biological activities and prevented ox-LDL induced cellular dysfunction in HUVECs.

摘要

为了了解中国蜂胶和巴西绿蜂胶调节血脂异常的作用机制，我们研究了它们对磷脂酰胆碱特异性磷脂酶 C（PC-PLC）活性和膜联蛋白 A7（ANXA7）水平的影响，这两者在控制动脉粥样硬化进展中起着至关重要的作用。此外，我们还研究了氧化型低密度脂蛋白（ox-LDL）刺激的人脐静脉内皮细胞（HUVEC）中的活性氧（ROS）水平、核因子-KappaB p65（NF- κ B p65）和线粒体膜电位（MMP）。我们的数据表明，两种类型的蜂胶 12.5μg/ml 处理均能显著提高细胞活力，降低细胞凋亡率，增加 ANXA7 水平，降低 PC-PLC 活性。两种蜂胶还能抑制 ROS 的产生以及随后的 MMP 崩溃，并抑制 ox-LDL 诱导的 HUVEC 中 NF- κ B p65 的激活。我们的结果还表明，中国蜂胶和巴西绿蜂胶具有相似的生物学活性，可防止 ox-LDL 诱导的 HUVEC 细胞功能障碍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/3e4227bf8397/ECAM2014-465383.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/57faa4e7a141/ECAM2014-465383.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/4082452c690f/ECAM2014-465383.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/1c026c3f3aa0/ECAM2014-465383.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/2aabea896e5f/ECAM2014-465383.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/f16909646945/ECAM2014-465383.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/3e4227bf8397/ECAM2014-465383.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/57faa4e7a141/ECAM2014-465383.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/4082452c690f/ECAM2014-465383.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/1c026c3f3aa0/ECAM2014-465383.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/2aabea896e5f/ECAM2014-465383.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/f16909646945/ECAM2014-465383.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c587/4016937/3e4227bf8397/ECAM2014-465383.006.jpg

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