School of Medicine, Department of Clinical Medicine, Institute of Molecular Medicine, Trinity College , Dublin 8, Ireland.
ACS Nano. 2014 Jun 24;8(6):5682-95. doi: 10.1021/nn500212h. Epub 2014 Jun 11.
Despite the widespread availability of immunohistochemical and other methodologies for screening and early detection of lung and breast cancer biomarkers, diagnosis of the early stage of cancers can be difficult and prone to error. The identification and validation of early biomarkers specific to lung and breast cancers, which would permit the development of more sensitive methods for detection of early disease onset, is urgently needed. In this paper, ultra-small and bright nanoprobes based on quantum dots (QDs) conjugated to single domain anti-HER2 (human epidermal growth factor receptor 2) antibodies (sdAbs) were applied for immunolabeling of breast and lung cancer cell lines, and their performance was compared to that of anti-HER2 monoclonal antibodies conjugated to conventional organic dyes Alexa Fluor 488 and Alexa Fluor 568. The sdAbs-QD conjugates achieved superior staining in a panel of lung cancer cell lines with differential HER2 expression. This shows their outstanding potential for the development of more sensitive assays for early detection of cancer biomarkers.
尽管免疫组织化学和其他方法广泛可用于筛选和早期检测肺癌和乳腺癌生物标志物，但癌症的早期诊断可能具有难度并且容易出错。因此，非常需要鉴定和验证专门针对肺癌和乳腺癌的早期生物标志物，这将允许开发更敏感的早期疾病检测方法。在本文中，应用基于量子点（QD）的超小而明亮的纳米探针与单域抗 HER2（人表皮生长因子受体 2）抗体（sdAb）偶联，对乳腺癌和肺癌细胞系进行免疫标记，并将其性能与与传统有机染料 Alexa Fluor 488 和 Alexa Fluor 568 偶联的抗 HER2 单克隆抗体进行比较。sdAb-QD 缀合物在具有不同 HER2 表达的肺癌细胞系中实现了优越的染色。这表明它们具有开发更敏感的癌症生物标志物早期检测分析方法的巨大潜力。
