Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait ; Queensland Children's Medical Research Institute, University of Queensland, Brisbane, QLD 4029, Australia.
Department of Biomedical Research, Dasman Diabetes Institute, 1180 Dasman, Kuwait.
Mediators Inflamm. 2014;2014:627150. doi: 10.1155/2014/627150. Epub 2014 Apr 17.
RANTES and its CCR5 receptor trigger inflammation and its progression to insulin resistance in obese. In the present study, we investigated for the first time the effect of physical exercise on the expression of RANTES and CCR5 in obese humans. Fifty-seven adult nondiabetic subjects (17 lean and 40 obese) were enrolled in a 3-month supervised physical exercise. RANTES and CCR5 expressions were measured in PBMCs and subcutaneous adipose tissue before and after exercise. Circulating plasma levels of RANTES were also investigated. There was a significant increase in RANTES and CCR5 expression in the subcutaneous adipose tissue of obese compared to lean. In PBMCs, however, while the levels of RANTES mRNA and protein were comparable between both groups, CCR5 mRNA was downregulated in obese subjects (P < 0.05). Physical exercise significantly reduced the expression of both RANTES and CCR5 (P < 0.05) in the adipose tissue of obese individuals with a concomitant decrease in the levels of the inflammatory markers TNF- α , IL-6, and P-JNK. Circulating RANTES correlated negatively with anti-inflammatory IL-1 ra (P = 0.001) and positively with proinflammatory IP-10 and TBARS levels (P < 0.05). Therefore, physical exercise may provide an effective approach for combating the deleterious effects associated with obesity through RANTES signaling in the adipose tissue.
RANTES 和其 CCR5 受体在肥胖者中引发炎症及其向胰岛素抵抗的进展。在本研究中，我们首次研究了体育锻炼对肥胖人群中 RANTES 和 CCR5 表达的影响。57 名成年非糖尿病受试者（17 名瘦人和 40 名肥胖者）参加了为期 3 个月的监督体育锻炼。在运动前后测量了 PBMC 和皮下脂肪组织中 RANTES 和 CCR5 的表达。还研究了循环血浆 RANTES 水平。与瘦人相比，肥胖者的皮下脂肪组织中 RANTES 和 CCR5 的表达显著增加。然而，在 PBMC 中，虽然两组之间 RANTES mRNA 和蛋白水平相当，但肥胖者的 CCR5 mRNA 下调（P < 0.05）。体育锻炼显著降低了肥胖者脂肪组织中 RANTES 和 CCR5 的表达（P < 0.05），同时炎症标志物 TNF-α、IL-6 和 P-JNK 的水平也降低了。循环 RANTES 与抗炎性 IL-1 ra 呈负相关（P = 0.001），与促炎性 IP-10 和 TBARS 水平呈正相关（P < 0.05）。因此，体育锻炼可能通过脂肪组织中的 RANTES 信号提供一种有效的方法来对抗与肥胖相关的有害影响。
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