Department of Biochemistry, Faculty of Science, University of Allahabad, Prayagraj 211002, India.
Department of Biology, College of Science, University of Hail, Hail, P.O. Box 2440, Saudi Arabia.
Biomed Res Int. 2022 Apr 28;2022:3892352. doi: 10.1155/2022/3892352. eCollection 2022.
possesses certain allelochemicals responsible for their medicinal effects. The presence of oils, polyphenols, alkaloids, terpenes, pseudoguaianolides, and histamines in has been shown to exhibit medicinal properties. However, the systematic biomedical properties of this plant are still unexplored. The extracts of leaves, stem, and flower of both at low and high temperatures (equivalent to boiling points of different solvents) were prepared. The extracts prepared in hexane, ethylacetate, methanol, and water were analyzed spectrophotometrically and colorimetrically and resolved on TLC for the presence of phytochemicals. The analyses of the free radical quenching potential of plant extracts were done by DPPH assay. The total antioxidant capacity was determined by phosphomolybdate assay and the ferric reducing antioxidant power (FRAP) assay was used to determine the reduction potential of the extracts. The spectrophotometric and qualitative analysis of plant extracts demonstrated the presence of alkaloids, terpenoids, carbohydrates, and cardiac glycosides. The occurrence of more than one Rf values for extracts determined by TLC indicated the presence of more than one phytochemical compound. The extracts contained strong antioxidant activity. These extracts exhibited strong antimicrobial activity against , , or , , and The evaluation of the antimicrobial potential of extracts was done by the disc diffusion method. These extracts also showed significant inhibition against HIV-1 RT activity. The anti-HIV-1 RT activity was done using Roche Kit. The extracts displayed the presence of many phytochemicals with strong antioxidant, antimicrobial, and anti-HIV-1 RT properties.
具有某些具有药用功效的次生物质。油、多酚、生物碱、萜类、伪愈创木烷内酯和组氨酸的存在已被证明具有药用特性。然而，这种植物的系统生物医学特性仍未被探索。已经制备了 和 的叶、茎和花的提取物，无论是在低温还是高温下（相当于不同溶剂的沸点）。用分光光度法和比色法分析了在己烷、乙酸乙酯、甲醇和水中制备的提取物，并在 TLC 上分析是否存在植物化学物质。通过 DPPH 测定法分析植物提取物的自由基猝灭潜力。通过磷钼酸盐测定法测定总抗氧化能力，并用铁还原抗氧化能力（FRAP）测定法测定提取物的还原电位。分光光度法和植物提取物的定性分析表明存在生物碱、萜类、碳水化合物和强心苷。TLC 确定的多个 Rf 值的提取物的存在表明存在多种植物化学物质。提取物具有很强的抗氧化活性。这些提取物对 、 、 或 、 和 表现出很强的抗菌活性。通过圆盘扩散法评估 提取物的抗菌潜力。这些提取物还对 HIV-1 RT 活性表现出显著抑制作用。使用罗氏试剂盒进行抗 HIV-1 RT 活性检测。提取物显示出存在许多具有强大抗氧化、抗菌和抗 HIV-1 RT 特性的植物化学物质。
