Cho Il-Hoon, Radadia Adarsh D, Farrokhzad Khashayar, Ximenes Eduardo, Bae Euiwon, Singh Atul K, Oliver Haley, Ladisch Michael, Bhunia Arun, Applegate Bruce, Mauer Lisa, Bashir Rashid, Irudayaraj Joseph
Bindley Bioscience and Birck Nanotechnology Center; Departments of.
Annu Rev Anal Chem (Palo Alto Calif). 2014;7:65-88. doi: 10.1146/annurev-anchem-071213-020249. Epub 2014 May 28.
Despite continuing research efforts, timely and simple pathogen detection with a high degree of sensitivity and specificity remains an elusive goal. Given the recent explosion of sensor technologies, significant strides have been made in addressing the various nuances of this important global challenge that affects not only the food industry but also human health. In this review, we provide a summary of the various ongoing efforts in pathogen detection and sample preparation in areas related to Fourier transform infrared and Raman spectroscopy, light scattering, phage display, micro/nanodevices, and nanoparticle biosensors. We also discuss the advantages and potential limitations of the detection methods and suggest next steps for further consideration.
尽管一直在进行研究，但要实现及时、简单且具有高度灵敏度和特异性的病原体检测，仍然是一个难以实现的目标。鉴于近期传感器技术的迅猛发展，在应对这一不仅影响食品行业而且关乎人类健康的重大全球挑战的各种细微差别方面已经取得了显著进展。在本综述中，我们总结了在傅里叶变换红外光谱和拉曼光谱、光散射、噬菌体展示、微/纳米器件以及纳米颗粒生物传感器等相关领域中，病原体检测和样品制备方面正在进行的各种努力。我们还讨论了检测方法的优点和潜在局限性，并提出了进一步考虑的后续步骤。
