Wu Zhaoying, Tang Tingting, Guo Han, Tang Songchao, Niu Yunfei, Zhang Jue, Zhang Wenjing, Ma Rui, Su Jiacan, Liu Changsheng, Wei Jie
Key Laboratory for Ultrafine Materials of Ministry of Education, East China University of Science and Technology, Shanghai 200237, PR China.
Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, PR China.
Colloids Surf B Biointerfaces. 2014 Aug 1;120:38-46. doi: 10.1016/j.colsurfb.2014.04.010. Epub 2014 May 22.
Mesoporous magnesium silicate (m-MS) was synthesized, and the in vitro degradability, bioactivity and primary cell responses to m-MS were investigated. The results suggested that the m-MS with mesoporous channels of approximately 5nm possessed the high specific surface area of 451.0m(2)/g and a large specific pore volume of 0.41cm(3)/g compared with magnesium silicate (MS) without mesopores of 75m(2)/g and 0.21cm(3)/g, respectively. The m-MS was able to absorb a large number of water, with water absorption of 74% compared with 26% for MS. The m-MS was also degradable in a Tris-HCl solution, with a weight loss ratio of 40wt% after a 70-day immersion period. The m-MS exhibited good in vitro bioactivity, inducing apatite formation on its surfaces after soaking in simulated body fluid (SBF) at a faster rate than observed for MS. The m-MS surface clearly promoted the proliferation and differentiation of MC3T3-E1 cells, and their normal cell morphology indicated excellent cytocompatibility. This study suggested that mesoporous magnesium silicate with a high specific surface area and pore volume had suitable degradability and good bioactivity and biocompatibility, making it an excellent candidate biomaterial for the induction of bone regeneration.
合成了介孔硅酸镁（m-MS），并研究了其体外降解性、生物活性以及对原代细胞的反应。结果表明，具有约5nm介孔通道的m-MS与无介孔的硅酸镁（MS）相比，具有451.0m²/g的高比表面积和0.41cm³/g的大比孔容，而MS的比表面积为75m²/g，比孔容为0.21cm³/g。m-MS能够吸收大量水分，吸水率为74%，而MS为26%。m-MS在Tris-HCl溶液中也可降解，浸泡70天后失重率为40wt%。m-MS在体外表现出良好的生物活性，浸泡在模拟体液（SBF）中后，其表面比MS更快地诱导磷灰石形成。m-MS表面明显促进了MC3T3-E1细胞的增殖和分化，其正常的细胞形态表明具有优异的细胞相容性。本研究表明，具有高比表面积和孔容的介孔硅酸镁具有合适的降解性、良好的生物活性和生物相容性，使其成为诱导骨再生的优秀候选生物材料。
