通过对海藻相关微生物群进行功能宏基因组学研究，鉴定并表征一种耐盐、冷活性海洋内切-β-1,4-葡聚糖酶。
Identification and characterization of a halotolerant, cold-active marine endo-β-1,4-glucanase by using functional metagenomics of seaweed-associated microbiota.
作者信息
Martin Marjolaine, Biver Sophie, Steels Sébastien, Barbeyron Tristan, Jam Murielle, Portetelle Daniel, Michel Gurvan, Vandenbol Micheline
机构信息
Microbiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium
Microbiology and Genomics Unit, Gembloux Agro-Bio Tech, University of Liège, Liège, Belgium.
出版信息
Appl Environ Microbiol. 2014 Aug;80(16):4958-67. doi: 10.1128/AEM.01194-14. Epub 2014 Jun 6.
Abstract
A metagenomic library was constructed from microorganisms associated with the brown alga Ascophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-β-1,4-glucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions.
摘要
从与褐藻泡叶藻相关的微生物构建了一个宏基因组文库。对该文库进行功能筛选，发现了13个新的假定酯酶基因座和2个糖苷水解酶基因座。序列和基因簇分析表明所鉴定的酶具有广泛的多样性，并让人了解了样本采集期间存在的微生物种群。最后，纯化并部分表征了一种与已知纤维素酶序列同一性低于50%的内切-β-1,4-葡聚糖酶，该酶在低温下以及在浓盐溶液中长时间孵育后仍具有活性。
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