Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (China).
Angew Chem Int Ed Engl. 2014 Jul 21;53(30):7809-13. doi: 10.1002/anie.201403918. Epub 2014 Jun 6.
Adenosine 5'-triphosphate (ATP) is a multifunctional molecule that participates in many important biological processes. Currently, fluorescence indicators for ATP with high performance are in demand. Reported herein is a novel water-soluble triarylboron compound which displays an apparent ATP-dependent fluorescence enhancement when dispersed in water. It can selectively recognize ATP from other bioactive substances in vitro and in vivo. The ATP-induced finite aggregation endows the indicator with appreciable photostability and superior tolerance to environmental electrolytes. This indicator has been successfully applied to the ATP imaging in NIH/3T3 fibroblast cells. The difference in the ATP levels within the membrane and cytosol is clearly visible.
三苯基硼是一种新型的水溶性三芳基硼化合物，在水中具有明显的 ATP 依赖性荧光增强效应，可选择性识别细胞内外的 ATP。这种 ATP 诱导的有限聚集赋予了该荧光探针优异的光稳定性和对环境电解质的耐受性。该探针已成功应用于 NIH/3T3 成纤维细胞内的 ATP 成像，清晰地显示了细胞膜和细胞质中 ATP 水平的差异。
