• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

小白菊内酯诱导亚马逊利什曼原虫无鞭毛体形式的细胞死亡。

Cell death in amastigote forms of Leishmania amazonensis induced by parthenolide.

作者信息

Tiuman Tatiana Shioji, Ueda-Nakamura Tânia, Alonso Antonio, Nakamura Celso Vataru

机构信息

Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá, Av, Colombo 5790, 87020-900 Maringá, Paraná, Brazil.

出版信息

BMC Microbiol. 2014 Jun 10;14:152. doi: 10.1186/1471-2180-14-152.

DOI:10.1186/1471-2180-14-152
PMID:24913205
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4067685/
Abstract

BACKGROUND

Leishmania amazonensis infection results in diverse clinical manifestations: cutaneous, mucocutaneous or visceral leishmaniasis. The arsenal of drugs available for treating Leishmania infections is limited. Therefore, new, effective, and less toxic leishmaniasis treatments are still needed. We verified cell death in amastigote forms of Leishmania amazonensis induced by the sesquiterpene lactone parthenolide.

RESULTS

The tested compound was able to concentration-dependently affect axenic and intracellular amastigotes, with IC50 values of 1.3 μM and 2.9 μM, respectively after 72 h incubation. No genotoxic effects were observed in a micronucleus test in mice. Parthenolide induced morphological and ultrastructural changes in axenic amastigotes, including a loss of membrane integrity, swelling of the mitochondrion, cytoplasmic vacuoles, and intense exocytic activity in the region of the flagellar pocket. These results led us to investigate the occurrence of autophagic vacuoles with monodansylcadaverine and the integrity of the plasma membrane and mitochondrial membrane potential using flow cytometry. In all of the tests, parthenolide had positive results.

CONCLUSIONS

Our results indicate that the antileishmanial action of parthenolide is associated with autophagic vacuole appearance, a reduction of fluidity, a loss of membrane integrity, and mitochondrial dysfunction. Considering the limited repertoire of existing antileishmanial compounds, the products derived from medicinal plants has been one the greatest advances to help develop new chemotherapeutic approaches.

摘要

背景

亚马逊利什曼原虫感染会导致多种临床表现：皮肤利什曼病、黏膜皮肤利什曼病或内脏利什曼病。可用于治疗利什曼原虫感染的药物种类有限。因此，仍然需要新的、有效且毒性较小的利什曼病治疗方法。我们验证了倍半萜内酯小白菊内酯诱导的亚马逊利什曼原虫无鞭毛体形式的细胞死亡。

结果

经测试的化合物能够浓度依赖性地影响体外培养和细胞内的无鞭毛体，孵育72小时后，其IC50值分别为1.3 μM和2.9 μM。在小鼠微核试验中未观察到遗传毒性作用。小白菊内酯诱导体外培养的无鞭毛体发生形态和超微结构变化，包括膜完整性丧失、线粒体肿胀、细胞质空泡形成以及鞭毛袋区域强烈的胞吐活性。这些结果促使我们使用单丹磺酰尸胺研究自噬泡的出现情况，并通过流式细胞术研究质膜完整性和线粒体膜电位。在所有测试中，小白菊内酯均得到阳性结果。

结论

我们的结果表明，小白菊内酯的抗利什曼原虫作用与自噬泡的出现、流动性降低、膜完整性丧失以及线粒体功能障碍有关。考虑到现有的抗利什曼原虫化合物种类有限，药用植物衍生产品一直是帮助开发新化疗方法的最大进展之一。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/446376c88387/1471-2180-14-152-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/1d6d72bb0451/1471-2180-14-152-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/0d523e1c480f/1471-2180-14-152-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/a751bfbbde15/1471-2180-14-152-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/3e6aeb4670da/1471-2180-14-152-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/6856f8c6fc3a/1471-2180-14-152-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/446376c88387/1471-2180-14-152-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/1d6d72bb0451/1471-2180-14-152-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/0d523e1c480f/1471-2180-14-152-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/a751bfbbde15/1471-2180-14-152-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/3e6aeb4670da/1471-2180-14-152-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/6856f8c6fc3a/1471-2180-14-152-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3588/4067685/446376c88387/1471-2180-14-152-6.jpg

相似文献

1
Cell death in amastigote forms of Leishmania amazonensis induced by parthenolide.小白菊内酯诱导亚马逊利什曼原虫无鞭毛体形式的细胞死亡。
BMC Microbiol. 2014 Jun 10;14:152. doi: 10.1186/1471-2180-14-152.
2
In vitro evaluation of (-)α-bisabolol as a promising agent against Leishmania amazonensis.（-）α-红没药醇作为抗亚马逊利什曼原虫潜在药物的体外评价
Exp Parasitol. 2015 Jan;148:66-72. doi: 10.1016/j.exppara.2014.10.001. Epub 2014 Nov 5.
3
Cell death and ultrastructural alterations in Leishmania amazonensis caused by new compound 4-Nitrobenzaldehyde thiosemicarbazone derived from S-limonene.源自 S-柠檬烯的新型化合物 4-硝基苯甲醛硫代半卡巴腙引起的亚马逊利什曼原虫的细胞死亡和超微结构改变
BMC Microbiol. 2014 Sep 26;14:236. doi: 10.1186/s12866-014-0236-0.
4
Antileishmanial activity of parthenolide, a sesquiterpene lactone isolated from Tanacetum parthenium.从艾菊中分离出的倍半萜内酯小白菊内酯的抗利什曼原虫活性。
Antimicrob Agents Chemother. 2005 Jan;49(1):176-82. doi: 10.1128/AAC.49.11.176-182.2005.
5
Antileishmanial activity of a 4-hydrazinoquinoline derivative: Induction of autophagy and apoptosis-related processes and effectiveness in experimental cutaneous leishmaniasis.4-肼基喹啉衍生物的抗利什曼原虫活性：诱导自噬和凋亡相关过程及其在实验性皮肤利什曼病中的疗效。
Exp Parasitol. 2018 Dec;195:78-86. doi: 10.1016/j.exppara.2018.10.007. Epub 2018 Oct 29.
6
A novel alkyl phosphocholine-dinitroaniline hybrid molecule exhibits biological activity in vitro against Leishmania amazonensis.一种新型的烷基膦胆碱-二硝苯胺杂化分子在体外对亚马逊利什曼原虫表现出生物活性。
Exp Parasitol. 2013 Sep;135(1):153-65. doi: 10.1016/j.exppara.2013.06.015. Epub 2013 Jul 8.
7
In vitro and in vivo antileishmania activity of sesquiterpene lactone-rich dichloromethane fraction obtained from Tanacetum parthenium (L.) Schultz-Bip.从艾菊（Tanacetum parthenium (L.) Schultz-Bip.）中获得的富含倍半萜内酯的二氯甲烷馏分的体外和体内抗利什曼原虫活性
Exp Parasitol. 2014 Aug;143:18-23. doi: 10.1016/j.exppara.2014.04.014. Epub 2014 May 5.
8
4-Quinolinylhydrazone analogues kill Leishmania (Leishmania) amazonensis by inducing apoptosis and mitochondria-dependent pathway cell death.4-喹啉基腙类似物通过诱导细胞凋亡和线粒体依赖性细胞死亡杀死美洲利什曼原虫（Leishmania） amazonensis。
Chem Biol Drug Des. 2024 May;103(5):e14535. doi: 10.1111/cbdd.14535.
9
Effects of (-) mammea A/BB isolated from Calophyllum brasiliense leaves and derivatives on mitochondrial membrane of Leishmania amazonensis.巴西叶中分离得到的（-）mammea A/BB 及其衍生物对亚马逊利什曼原虫线粒体膜的影响。
Phytomedicine. 2012 Feb 15;19(3-4):223-30. doi: 10.1016/j.phymed.2011.10.008. Epub 2012 Jan 28.
10
Eugenia piauhiensis Vellaff. essential oil and γ-elemene its major constituent exhibit antileishmanial activity, promoting cell membrane damage and in vitro immunomodulation.薇拉芳油及其主要成分γ-榄香烯具有抗利什曼原虫活性，可促进细胞膜损伤和体外免疫调节。
Chem Biol Interact. 2021 Apr 25;339:109429. doi: 10.1016/j.cbi.2021.109429. Epub 2021 Mar 10.

引用本文的文献

1
Natural-Product-Based Solutions for Tropical Infectious Diseases.天然产物在热带传染病防治中的应用。
Clin Microbiol Rev. 2021 Dec 15;34(4):e0034820. doi: 10.1128/CMR.00348-20. Epub 2021 Sep 8.
2
Asteraceae Plants as Sources of Compounds Against Leishmaniasis and Chagas Disease.菊科植物作为抗利什曼病和恰加斯病化合物的来源。
Front Pharmacol. 2019 May 8;10:477. doi: 10.3389/fphar.2019.00477. eCollection 2019.
3
Insights into Chagas treatment based on the potential of bacteriocin AS-48.基于细菌素 AS-48 的潜力对恰加斯病治疗的深入了解。

本文引用的文献

1
Recent advances in leishmaniasis treatment.利什曼病治疗的最新进展。
Int J Infect Dis. 2011 Aug;15(8):e525-32. doi: 10.1016/j.ijid.2011.03.021. Epub 2011 May 24.
2
Plant derived therapeutics for the treatment of Leishmaniasis.植物来源的疗法治疗利什曼病。
Phytomedicine. 2011 Sep 15;18(12):1056-69. doi: 10.1016/j.phymed.2011.03.004. Epub 2011 May 18.
3
Amphotericin B membrane action: role for two types of ion channels in eliciting cell survival and lethal effects.两性霉素 B 的膜作用：两种类型的离子通道在引发细胞存活和致死效应中的作用。
Int J Parasitol Drugs Drug Resist. 2019 Aug;10:1-8. doi: 10.1016/j.ijpddr.2019.03.003. Epub 2019 Mar 29.
4
Leishmanicidal activity of α-bisabolol from Tunisian chamomile essential oil.突尼斯洋甘菊精油中α-红没药醇的杀利什曼原虫活性。
Parasitol Res. 2018 Sep;117(9):2855-2867. doi: 10.1007/s00436-018-5975-7. Epub 2018 Jun 28.
5
Isopropyl quinoxaline-7-carboxylate 1,4-di-N-oxide derivatives induce regulated necrosis-like cell death on Leishmania (Leishmania) mexicana.喹喔啉-7-羧酸异丙酯1,4-二-N-氧化物衍生物可诱导墨西哥利什曼原虫（利什曼原虫属）发生类程序性坏死样细胞死亡。
Parasitol Res. 2018 Jan;117(1):45-58. doi: 10.1007/s00436-017-5635-3. Epub 2017 Nov 20.
6
Investigation of the Anti-Leishmania (Leishmania) infantum Activity of Some Natural Sesquiterpene Lactones.某些天然倍半萜内酯对婴儿利什曼原虫（利什曼原虫）的抗虫活性研究
Molecules. 2017 Apr 25;22(5):685. doi: 10.3390/molecules22050685.
7
Computer-Aided Drug Design Using Sesquiterpene Lactones as Sources of New Structures with Potential Activity against Infectious Neglected Diseases.利用倍半萜内酯作为具有抗被忽视传染病潜在活性的新型结构来源的计算机辅助药物设计
Molecules. 2017 Jan 3;22(1):79. doi: 10.3390/molecules22010079.
8
Flavonoids and Sesquiterpene Lactones from and against Worms.来自[具体来源未给出]的黄酮类化合物和倍半萜内酯对蠕虫的作用。
Evid Based Complement Alternat Med. 2016;2016:9521349. doi: 10.1155/2016/9521349. Epub 2016 Nov 17.
9
Three new trixane glycosides obtained from the leaves of Jungia sellowii Less. using centrifugal partition chromatography.利用离心分配色谱法从黄氏容吉草（Jungia sellowii Less.）叶片中获得了三种新的三亚甲基己烷糖苷。
Beilstein J Org Chem. 2016 Apr 12;12:674-83. doi: 10.3762/bjoc.12.68. eCollection 2016.
10
Cell death and ultrastructural alterations in Leishmania amazonensis caused by new compound 4-Nitrobenzaldehyde thiosemicarbazone derived from S-limonene.源自 S-柠檬烯的新型化合物 4-硝基苯甲醛硫代半卡巴腙引起的亚马逊利什曼原虫的细胞死亡和超微结构改变
BMC Microbiol. 2014 Sep 26;14:236. doi: 10.1186/s12866-014-0236-0.
J Membr Biol. 2010 Dec;238(1-3):1-20. doi: 10.1007/s00232-010-9313-y. Epub 2010 Nov 18.
4
Safety evaluation of proanthocyanidin polymer-rich fraction obtained from stem bark of Stryphnodendron adstringens (BARBATIMAO) for use as a pharmacological agent.从 Stryphnodendron adstringens（BARBATIMAO）树皮中提取的原花青素聚合物富分作为药理学制剂的安全性评价。
Regul Toxicol Pharmacol. 2010 Nov;58(2):330-5. doi: 10.1016/j.yrtph.2010.07.006. Epub 2010 Jul 23.
5
The effects on Trypanosoma cruzi of novel synthetic naphthoquinones are mediated by mitochondrial dysfunction.新型合成萘醌对克氏锥虫的作用是由线粒体功能障碍介导的。
Free Radic Biol Med. 2009 Sep 1;47(5):644-53. doi: 10.1016/j.freeradbiomed.2009.06.004. Epub 2009 Jun 6.
6
Atypical disseminated leishmaniasis similar to post-kala-azar dermal leishmaniasis in a Brazilian AIDS patient infected with Leishmania (Leishmania) infantum chagasi: a case report.巴西 AIDS 患者感染恰加斯原虫（Leishmania）引起的非典型播散性黑热病类似皮肤利什曼病：1 例报告。
Int J Infect Dis. 2009 Nov;13(6):e504-7. doi: 10.1016/j.ijid.2009.01.022. Epub 2009 May 17.
7
Leishmaniasis: drug resistance and natural products (review).利什曼病：耐药性与天然产物（综述）
Int J Mol Med. 2008 Sep;22(3):277-86.
8
Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes.高等真核生物中自噬监测检测方法的使用与解读指南。
Autophagy. 2008 Feb;4(2):151-75. doi: 10.4161/auto.5338. Epub 2007 Nov 21.
9
Terpenes increase the partitioning and molecular dynamics of an amphipathic spin label in stratum corneum membranes.萜烯增加了角质层膜中两亲性自旋标记物的分配和分子动力学。
Int J Pharm. 2008 Feb 28;350(1-2):103-12. doi: 10.1016/j.ijpharm.2007.08.024. Epub 2007 Aug 24.
10
Genetic diversity of Leishmania amazonensis strains isolated in northeastern Brazil as revealed by DNA sequencing, PCR-based analyses and molecular karyotyping.通过DNA测序、基于PCR的分析和分子核型分析揭示的巴西东北部分离的亚马逊利什曼原虫菌株的遗传多样性。
Kinetoplastid Biol Dis. 2007 Jun 21;6:5. doi: 10.1186/1475-9292-6-5.