Department of Biochemistry, School of Life Sciences, Vels University, Pallavaram, Chennai 600117, India.
J Ethnopharmacol. 2011 Apr 12;134(3):1024-7. doi: 10.1016/j.jep.2011.01.010. Epub 2011 Jan 18.
To investigate the anti-inflammatory and antioxidant potential of ethanolic extract of Leucas aspera (EELA) in adjuvant arthritis.
Complete Freund's adjuvant served to induce arthritis. EELA was administered in two doses along with vehicle control (0.1% carboxymethyl cellulose) and positive control (Diclofenac). Levels of tumour necrosis factor (TNF-)-α, C-reactive protein (CRP), Interleukin-2 (IL-2), Cathepsin D, activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were estimated in plasma/hemolysate and tissue. HPLC analysis of EELA was also performed.
EELA exhibited significant anti-inflammatory (p<0.001) and antioxidant activity (p<0.001). It did not show mortality up to 2000 mg/kg body weight. Histopathological studies confirmed complete cartilage regeneration and near normal joint in EELA2 treated arthritic rats.
This study highlighted the antioxidant and anti-inflammatory potential of Leucas aspera. Three major families of compounds present in EELA may explain these activities: catechins (epicatechin, beta epicatechin), flavonoids (procyanidin), phytosterols (beta-sitosterol) apart from glycosides, phenolic compounds and tannins.
研究 Leucas aspera（EELA）的乙醇提取物的抗炎和抗氧化潜力在佐剂性关节炎中的作用。
完全弗氏佐剂用于诱导关节炎。EELA 以两种剂量给药，同时给予载体对照（0.1%羧甲基纤维素）和阳性对照（双氯芬酸）。测定血浆/溶血物和组织中肿瘤坏死因子（TNF-α）、C 反应蛋白（CRP）、白细胞介素-2（IL-2）、组织蛋白酶 D、抗氧化酶超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）的活性。还对 EELA 进行了 HPLC 分析。
EELA 表现出显著的抗炎（p<0.001）和抗氧化活性（p<0.001）。它在 2000 毫克/千克体重以下没有表现出致死性。组织病理学研究证实 EELA2 治疗的关节炎大鼠完全再生软骨和接近正常关节。
本研究强调了 Leucas aspera 的抗氧化和抗炎潜力。EELA 中存在的三个主要化合物家族：儿茶素（表儿茶素、β-表儿茶素）、类黄酮（原花青素）、植物甾醇（β-谷甾醇），除了糖苷、酚类化合物和单宁外，可能解释了这些活性。
