Li Yan, Liu Xiang-Yong, Ma Ming-Ming, Qi Zhi-Jiang, Zhang Xiao-Qiang, Li Zhi, Cao Guo-Hong, Li Jun, Zhu Wei-Wei, Wang Xiao-Zhi
Yan Li, Ming-Ming Ma, Zhi-Jiang Qi, Zhi Li, Guo-Hong Cao, Jun Li, Wei-Wei Zhu, Xiao-Zhi Wang, Department of Respiratory Medicine and Intensive Care Unit, Affiliated Hospital of Binzhou Medical University, Binzhou 256603, Shandong Province, China.
World J Gastroenterol. 2014 May 21;20(19):5849-58. doi: 10.3748/wjg.v20.i19.5849.
To implement high-throughput 16S rDNA sequencing to study microbial diversity in the fecal matter of rats with acute lung injury/acute respiratory distress syndrome (ALI/ARDS).
Intratracheal instillation of lipopolysaccharide was used to induce ALI, and the pathological changes in the lungs and intestines were observed. D-lactate levels and diamine oxidase (DAO) activities were determined by enzymatic spectrophotometry. The fragments encompassing V4 16S rDNA hypervariable regions were PCR amplified from fecal samples, and the PCR products of V4 were sequenced by Illumina MiSeq.
Increased D-lactate levels and DAO activities were observed in the model group (P < 0.01). Sequencing results revealed the presence of 3780 and 4142 species in the control and model groups, respectively. The percentage of shared species was 18.8419%. Compared with the control group, the model group had a higher diversity index and a lower number of species of Fusobacteria (at the phylum level), Helicobacter and Roseburia (at the genus level) (P < 0.01). Differences in species diversity, structure, distribution and composition were found between the control group and early ARDS group.
The detection of specific bacteria allows early detection and diagnosis of ALI/ARDS.
采用高通量16S rDNA测序技术研究急性肺损伤/急性呼吸窘迫综合征（ALI/ARDS）大鼠粪便中的微生物多样性。
采用气管内滴注脂多糖诱导ALI，观察肺和肠道的病理变化。采用酶分光光度法测定D-乳酸水平和二胺氧化酶（DAO）活性。从粪便样本中PCR扩增包含V4 16S rDNA高变区的片段，并通过Illumina MiSeq对V4的PCR产物进行测序。
模型组D-乳酸水平和DAO活性升高（P < 0.01）。测序结果显示，对照组和模型组分别存在3780种和4142种。共有物种的百分比为18.8419%。与对照组相比，模型组的多样性指数更高，梭杆菌门（在门水平）、幽门螺杆菌属和罗斯氏菌属（在属水平）的物种数量更低（P < 0.01）。对照组和早期ARDS组之间在物种多样性、结构、分布和组成方面存在差异。
检测特定细菌有助于早期检测和诊断ALI/ARDS。
