Ajdaharian Janet, Dadkhah Mohammad, Sabokpey Sara, Biren-Fetz John, Chung Na Eun, Wink Cherie, Wilder-Smith Petra
Beckman Laser Institute, University of California, Irvine, California, 92612.
Lasers Surg Med. 2014 Sep;46(7):546-52. doi: 10.1002/lsm.22265. Epub 2014 Jun 10.
Oral biofilm formation and progression on the surface of the tooth can lead to advanced oral disease such as gingivitis. The purpose of this randomized, controlled, double-blinded study was to evaluate the effects of a novel dental gel on oral plaque biofilm using multimodal imaging techniques.
Twenty-five subjects with moderate gingival inflammation (Löe and Silness Gingival Index ≥2) and pocket depths <4 were randomly assigned to brush twice daily for 21 days with the test or the control dental gel. In vivo multimodality in situ imaging was performed over a 3-week period using in vivo Optical Coherence Tomography (OCT) and Non-Linear Optical microscopy (NLOM). Plaque levels, gingival inflammation and gingival bleeding were also charted on days 0, 7, 14, and 21 using standard clinical indices.
After 3 weeks, OCT and NLOM images showed a macroscopic break-up of the plaque layer and smaller, fragmented residual deposits in the test group with no apparent changes in the pellicle. Biofilm was also reduced in the control group, but to a lesser degree with regard to thickness, continuity and surface area. Paralleling these imaging results, clinical indices were significantly improved in both groups (P < 0.05) and significantly lower in the test group (P < 0.05).
Both dental gels reduced oral biofilm with the test gel showing greater efficacy (P < 0.05) as determined by clinical and imaging parameters.
牙齿表面的口腔生物膜形成与进展可导致如牙龈炎等晚期口腔疾病。这项随机、对照、双盲研究的目的是使用多模态成像技术评估一种新型牙科凝胶对口腔菌斑生物膜的影响。
25名患有中度牙龈炎症（洛和西伦斯牙龈指数≥2）且牙周袋深度<4的受试者被随机分配，每天使用测试或对照牙科凝胶刷牙两次，持续21天。在3周内使用体内光学相干断层扫描（OCT）和非线性光学显微镜（NLOM）进行体内多模态原位成像。在第0、7、14和21天，还使用标准临床指标记录菌斑水平、牙龈炎症和牙龈出血情况。
3周后，OCT和NLOM图像显示测试组中菌斑层出现宏观破裂，残留沉积物更小且呈碎片状，而薄膜无明显变化。对照组的生物膜也有所减少，但在厚度、连续性和表面积方面减少程度较小。与这些成像结果一致，两组的临床指标均有显著改善（P < 0.05），且测试组显著更低（P < 0.05）。
两种牙科凝胶均能减少口腔生物膜，根据临床和成像参数，测试凝胶显示出更高的疗效（P < 0.05）。
