Garmendia-Torres Cecilia, Skupin Alexander, Michael Sean A, Ruusuvuori Pekka, Kuwada Nathan J, Falconnet Didier, Cary Gregory A, Hansen Carl, Wiggins Paul A, Dudley Aimée M
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.
Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-sur-Alzette, Luxembourg; National Center for Microscopy and Imaging Research, University of California San Diego, La Jolla, California, United States of America.
PLoS One. 2014 Jun 11;9(6):e99428. doi: 10.1371/journal.pone.0099428. eCollection 2014.
P-bodies belong to a large family of RNA granules that are associated with post-transcriptional gene regulation, conserved from yeast to mammals, and influence biological processes ranging from germ cell development to neuronal plasticity. RNA granules can also transport RNAs to specific locations. Germ granules transport maternal RNAs to the embryo, and neuronal granules transport RNAs long distances to the synaptic dendrites. Here we combine microfluidic-based fluorescent microscopy of single cells and automated image analysis to follow p-body dynamics during cell division in yeast. Our results demonstrate that these highly dynamic granules undergo a unidirectional transport from the mother to the daughter cell during mitosis as well as a constrained "hovering" near the bud site half an hour before the bud is observable. Both behaviors are dependent on the Myo4p/She2p RNA transport machinery. Furthermore, single cell analysis of cell size suggests that PBs play an important role in daughter cell growth under nutrient limiting conditions.
P小体属于一大类RNA颗粒，与转录后基因调控相关，从酵母到哺乳动物都保守存在，并影响从生殖细胞发育到神经元可塑性等一系列生物学过程。RNA颗粒还能将RNA转运到特定位置。生殖颗粒将母体RNA转运到胚胎，神经元颗粒将RNA远距离转运到突触树突。在这里，我们结合基于微流控的单细胞荧光显微镜技术和自动图像分析，来追踪酵母细胞分裂过程中P小体的动态变化。我们的结果表明，这些高度动态的颗粒在有丝分裂期间从母细胞向子细胞进行单向运输，并且在芽出现前半小时在芽位点附近进行受限的“徘徊”。这两种行为都依赖于Myo4p/She2p RNA转运机制。此外，对细胞大小的单细胞分析表明，在营养限制条件下，PBs在子细胞生长中起重要作用。
