Piubeli Francine, Grossman Matthew J, Fantinatti-Garboggini Fabiana, Durrant Lucia R
Department of Food Science (DCA), Food Engineering Faculty (FEA), University of Campinas (UNICAMP), Rua Monteiro Lobato, 80, Campinas, SP, CEP 13083-862, Brazil.
Environ Sci Pollut Res Int. 2014 Oct;21(20):12006-16. doi: 10.1007/s11356-014-3155-6. Epub 2014 Jun 13.
In this work the archaea and eubacteria community of a hypersaline produced water from the Campos Basin that had been transported and discharged to an onshore storage facility was evaluated by 16S recombinant RNA (rRNA) gene sequence analysis. The produced water had a hypersaline salt content of 10 (w/v), had a carbon oxygen demand (COD) of 4,300 mg/l and contains phenol and other aromatic compounds. The high salt and COD content and the presence of toxic phenolic compounds present a problem for conventional discharge to open seawater. In previous studies, we demonstrated that the COD and phenolic content could be largely removed under aerobic conditions, without dilution, by either addition of phenol degrading Haloarchaea or the addition of nutrients alone. In this study our goal was to characterize the microbial community to gain further insight into the persistence of reservoir community members in the produced water and the potential for bioremediation of COD and toxic contaminants. Members of the archaea community were consistent with previously identified communities from mesothermic reservoirs. All identified archaea were located within the phylum Euryarchaeota, with 98 % being identified as methanogens while 2 % could not be affiliated with any known genus. Of the identified archaea, 37 % were identified as members of the strictly carbon-dioxide-reducing genus Methanoplanus and 59 % as members of the acetoclastic genus Methanosaeta. No Haloarchaea were detected, consistent with the need to add these organisms for COD and aromatic removal. Marinobacter and Halomonas dominated the eubacterial community. The presence of these genera is consistent with the ability to stimulate COD and aromatic removal with nutrient addition. In addition, anaerobic members of the phyla Thermotogae, Firmicutes, and unclassified eubacteria were identified and may represent reservoir organisms associated with the conversion hydrocarbons to methane.
在本研究中，通过16S重组RNA（rRNA）基因序列分析，对来自坎波斯盆地的高盐采出水的古菌和真细菌群落进行了评估，该采出水已被输送并排放到岸上储存设施。采出水的盐含量高达10%（w/v），化学需氧量（COD）为4300mg/l，含有苯酚和其他芳香族化合物。高盐和COD含量以及有毒酚类化合物的存在给传统的向开放海水排放带来了问题。在先前的研究中，我们证明了通过添加降解苯酚的嗜盐古菌或仅添加营养物质，在有氧条件下无需稀释即可大量去除COD和酚类含量。在本研究中，我们的目标是对微生物群落进行表征，以进一步了解储层群落成员在采出水中的持久性以及COD和有毒污染物的生物修复潜力。古菌群落的成员与先前从中温储层中鉴定出的群落一致。所有鉴定出的古菌都属于广古菌门，其中98%被鉴定为产甲烷菌，而2%无法与任何已知属相关联。在鉴定出的古菌中，37%被鉴定为严格还原二氧化碳的甲烷盘菌属成员，59%为乙酸裂解型甲烷八叠球菌属成员。未检测到嗜盐古菌，这与添加这些生物体以去除COD和芳香族化合物的需求一致。海杆菌属和嗜盐单胞菌属在真细菌群落中占主导地位。这些属的存在与添加营养物质刺激去除COD和芳香族化合物的能力一致。此外，还鉴定出了栖热袍菌门、厚壁菌门和未分类真细菌的厌氧成员，它们可能代表与将碳氢化合物转化为甲烷相关的储层生物。
