什么时候 2 加 2 等于 5？抗菌药物协同作用检测的回顾。
When does 2 plus 2 equal 5? A review of antimicrobial synergy testing.
机构信息
Virginia Commonwealth University Medical Center, Richmond, Virginia, USA
出版信息
J Clin Microbiol. 2014 Dec;52(12):4124-8. doi: 10.1128/JCM.01121-14. Epub 2014 Jun 11.
Abstract
In this age of emerging antibiotic resistance, limited therapeutic options exist for treating multidrug-resistant organisms. Combination therapy is commonly employed to manage these infections despite little laboratory guidance as to the efficacy of this approach. Synergy testing methods have been used to assess the interaction of antibiotic combinations in vitro. This review will discuss the four primary methods used to assess synergy, as well as the data that exist for testing of cystic fibrosis. In the final analysis, this review concludes that there is not enough evidence to endorse synergy testing for routine clinical use.
摘要
在这个抗生素耐药性不断出现的时代，治疗多重耐药菌的治疗选择有限。尽管实验室对这种方法的疗效几乎没有指导，但联合治疗通常用于治疗这些感染。协同测试方法已被用于评估抗生素组合在体外的相互作用。这篇综述将讨论用于评估协同作用的四种主要方法，以及存在的用于测试囊性纤维化的协同作用的数据。总的来说，这篇综述的结论是，没有足够的证据支持将协同作用测试常规用于临床。
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Expert Rev Respir Med. 2013 Aug;7(4):385-96. doi: 10.1586/17476348.2013.814411.
2
Combination therapy for treatment of infections with gram-negative bacteria.联合治疗用于治疗革兰氏阴性菌感染。
Clin Microbiol Rev. 2012 Jul;25(3):450-70. doi: 10.1128/CMR.05041-11.
3
Cystic fibrosis pulmonary guidelines: treatment of pulmonary exacerbations.囊性纤维化肺部指南：肺部急性加重的治疗
Am J Respir Crit Care Med. 2009 Nov 1;180(9):802-8. doi: 10.1164/rccm.200812-1845PP. Epub 2009 Sep 3.
4
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5
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7
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8
In vitro synergy of daptomycin plus rifampin against Enterococcus faecium resistant to both linezolid and vancomycin.达托霉素联合利福平对耐利奈唑胺和万古霉素的屎肠球菌的体外协同作用
Antimicrob Agents Chemother. 2005 Dec;49(12):5166-8. doi: 10.1128/AAC.49.12.5166-5168.2005.
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Combination antibiotic susceptibility testing to treat exacerbations of cystic fibrosis associated with multiresistant bacteria: a randomised, double-blind, controlled clinical trial.联合抗生素敏感性试验治疗多重耐药菌相关的囊性纤维化急性加重：一项随机、双盲、对照临床试验。
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