Ma Shengli, Li Hui, Yan Chuang, Wang Dan, Li Haiqing, Xia Xue, Dong Xue, Zhao Yingnan, Sun Tingting, Hu Pengfei, Guan Weijun
Department of Stomatology, Hospital of Heilongjiang Province, Harbin, Heilongjiang 150036, P.R. China.
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R. China.
Exp Ther Med. 2014 Jun;7(6):1486-1494. doi: 10.3892/etm.2014.1618. Epub 2014 Mar 12.
An antibacterial substance from () is known to have an inhibitory effect on putative periodontal pathogens, but its inhibitory effect on pathogens of oral candidiasis is unknown. In this study, intracellular and exocrine proteins were extracted from . The antagonistic effect of the protein extracts on () and () was detected by a well-plate technique, and the effects of the protein extracts on biofilms formed by these bacteria were evaluated by confocal laser scanning microscopy. The antagonistic effect of the protein extracts on pathogenic fungi was investigated using () and (). The growth curves of and were determined from ultraviolet absorption measurements, their morphological changes following treatment were observed by optical microscopy and scanning electron microscopy, and the effects of the protein extracts on the thickness of their biofilms and the distribution of dead/live bacteria within the biofilms were detected by confocal laser scanning microscopy. The results showed significant inhibitory effects of the intracellular proteins extracted from on pathogenic bacteria ( and ), fungi ( and ) and the biofilms formed by them. Furthermore, the growth curves and morphology of and were altered following treatment with the intracellular proteins, resulting in disc-like depressions in the surfaces of the fungal spores and mycelia. By contrast, the exocrine proteins demonstrated no significant inhibitory effect on the pathogenic bacteria, fungi and the biofilms formed by them. Thus, it may be concluded that intracellular proteins of have antibacterial activity and exert an antagonistic effect on certain pathogenic bacteria and fungi of the oral cavity.
已知来自（）的一种抗菌物质对假定的牙周病原体具有抑制作用，但其对口腔念珠菌病病原体的抑制作用尚不清楚。在本研究中，从（）中提取细胞内和外分泌蛋白。采用微孔板技术检测蛋白提取物对（）和（）的拮抗作用，并通过共聚焦激光扫描显微镜评估蛋白提取物对这些细菌形成的生物膜的影响。使用（）和（）研究蛋白提取物对致病真菌的拮抗作用。通过紫外吸收测量确定（）和（）的生长曲线，通过光学显微镜和扫描电子显微镜观察处理后它们的形态变化，并通过共聚焦激光扫描显微镜检测蛋白提取物对其生物膜厚度以及生物膜内死/活细菌分布的影响。结果表明，从（）中提取的细胞内蛋白对致病细菌（和）、真菌（和）及其形成的生物膜具有显著抑制作用。此外，用细胞内蛋白处理后，（）和（）的生长曲线和形态发生改变，导致真菌孢子和菌丝体表面出现盘状凹陷。相比之下，外分泌蛋白对致病细菌、真菌及其形成的生物膜没有显著抑制作用。因此，可以得出结论，（）的细胞内蛋白具有抗菌活性，并对口腔中的某些致病细菌和真菌发挥拮抗作用。
