Wang Desheng, Schreurs Bernard G
Department of Physiology and Pharmacology, West Virginia University School of Medicine, Morgantown, West Virginia, 26506; Blanchette Rockefeller Neurosciences Institute, Morgantown, West Virginia, 26505.
Dev Neurobiol. 2014 Dec;74(12):1268-76. doi: 10.1002/dneu.22203. Epub 2014 Jun 26.
Patch clamp recordings of neurons in the adult rat deep cerebellar nuclei have been limited by the availability of viable brain slices. Using a new slicing technique, this study was designed to explore the maturation of membrane properties of neurons in the deep cerebellar nuclei (DCN)-an area involved in rat eyeblink conditioning. Compared to whole-cell current-clamp recordings in DCN in rat pups at postnatal day 16 (P16) to P21, recordings from weanling rats at P22-P40 revealed a number of significant changes including an increase in the amplitude of the afterhyperpolarization (AHP)-an index of membrane excitability which has been shown to be important for eyeblink conditioning-a prolonged interval between the first and second evoked action potential, and an increase in AHP amplitude for hyperpolarization-induced rebound spikes. This is the first report of developmental changes in membrane properties of DCN which may contribute to the ontogeny of eyeblink conditioning in the rat.
成年大鼠小脑深部核团神经元的膜片钳记录一直受到可行脑片可用性的限制。本研究采用一种新的切片技术,旨在探索小脑深部核团(DCN)中神经元膜特性的成熟过程,DCN是一个与大鼠眨眼条件反射有关的区域。与出生后第16天(P16)至第21天的幼鼠DCN全细胞电流钳记录相比,出生后第22天至第40天断奶大鼠的记录显示出许多显著变化,包括超极化后电位(AHP)幅度增加——AHP是膜兴奋性的指标,已被证明对眨眼条件反射很重要——第一个和第二个诱发动作电位之间的间隔延长,以及超极化诱导的反弹尖峰的AHP幅度增加。这是关于DCN膜特性发育变化的首次报道,这些变化可能有助于大鼠眨眼条件反射的个体发生。