Tousch Didier, Bidel Luc P R, Cazals Guillaume, Ferrare Karine, Leroy Jeremy, Faucanié Marie, Chevassus Hugues, Tournier Michel, Lajoix Anne-Dominique, Azay-Milhau Jacqueline
Université Montpellier I , 4 Boulevard Henri IV, Montpellier, France.
J Agric Food Chem. 2014 Aug 6;62(31):7738-45. doi: 10.1021/jf500926v. Epub 2014 Jul 23.
In the present study, we obtained a dried burdock root extract (DBRE) rich in caffeoylquinic acids derivatives. We performed the chemical characterization of DBRE and explored its antihyperglycemic potential in both in vitro and in vivo experiments. Chemical analysis of DBRE using LC-MS and GC-MS revealed the presence of a great majority of dicaffeoylquinic acid derivatives (75.4%) of which 1,5-di-O-caffeoyl-4-O-maloylquinic acid represents 44% of the extract. In the in vitro experiments, DBRE is able to increase glucose uptake in cultured L6 myocytes and to decrease glucagon-induced glucose output from rat isolated hepatocytes together with a reduction of hepatic glucose 6-phosphatase activity. DBRE did not increase insulin secretion in the INS-1 pancreatic β-cell line. In vivo, DBRE improves glucose tolerance both after intraperitoneal and oral subchronic administration. In conclusion, our data demonstrate that DBRE constitutes an original set of caffeoylquinic acid derivatives displaying antihyperglycemic properties.
在本研究中，我们获得了一种富含咖啡酰奎宁酸衍生物的牛蒡根干提取物（DBRE）。我们对DBRE进行了化学表征，并在体外和体内实验中探索了其降血糖潜力。使用液相色谱-质谱联用（LC-MS）和气相色谱-质谱联用（GC-MS）对DBRE进行化学分析，结果显示存在绝大多数二咖啡酰奎宁酸衍生物（75.4%），其中1,5-二-O-咖啡酰-4-O-苹果酰奎宁酸占提取物的44%。在体外实验中，DBRE能够增加培养的L6肌细胞对葡萄糖的摄取，并减少胰高血糖素诱导的大鼠分离肝细胞的葡萄糖输出，同时降低肝葡萄糖6-磷酸酶活性。DBRE在INS-1胰腺β细胞系中不会增加胰岛素分泌。在体内，腹腔内和口服亚慢性给药后，DBRE均可改善葡萄糖耐量。总之，我们的数据表明，DBRE是一组具有降血糖特性的独特咖啡酰奎宁酸衍生物。
