Joycharat Nantiya, Thammavong Sonesay, Voravuthikunchai Supayang Piyawan, Plodpai Patimaporn, Mitsuwan Watcharapong, Limsuwan Surasak, Subhadhirasakul Sanan
a Faculty of Traditional Thai Medicine, Prince of Songkla University , Songkhla 90112 , Thailand.
Nat Prod Res. 2014;28(23):2169-72. doi: 10.1080/14786419.2014.924934. Epub 2014 Jun 17.
The stem-derived essential oil of Aglaia odorata Lour. was obtained by hydrodistillation using a Clevenger-type system. Gas chromatography-mass spectrometry analysis of the oil revealed the identification of 39 compounds, representing 76.4% of the oil; germacrene D (20.3%), α-humulene (17.1%), α-himachalene (12.7%) and β-caryophyllene (10.2%) were the major components. Ar-turmerone (1) and eichlerialactone (2) were isolated from the stem oil and ethanolic stem extract of this plant species, respectively. Antimicrobial activities of the oil and ethanol extract were tested against both Gram-positive and Gram-negative bacterial strains including Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923, Acinetobacter baumannii ATCC 19606 and Escherichia coli ATCC 25922, as well as three rice fungal pathogens Bipolaris oryzae, Pyricularia oryzae and Rhizoctonia solani using broth microdilution method. The oil and 1 exhibited significant antifungal activity against the three rice pathogens tested, whereas 2 exhibited good antibacterial activity against both the Gram-positive pathogens tested.
采用克莱文杰型装置通过水蒸馏法从米仔兰茎中提取挥发油。气相色谱-质谱联用分析表明，该挥发油中鉴定出39种化合物，占挥发油总量的76.4%；主要成分包括杜松烯D（20.3%）、α-葎草烯（17.1%）、α-雪松烯（12.7%）和β-石竹烯（10.2%）。分别从该植物的茎挥发油和乙醇提取物中分离得到莪术酮（1）和艾氏内酯（2）。采用肉汤微量稀释法，检测了该挥发油和乙醇提取物对包括蜡样芽孢杆菌ATCC 11778、金黄色葡萄球菌ATCC 25923、鲍曼不动杆菌ATCC 19606和大肠埃希菌ATCC 25922在内的革兰氏阳性和革兰氏阴性细菌菌株以及三种水稻真菌病原体稻瘟病菌、稻瘟病菌和立枯丝核菌的抗菌活性。该挥发油和化合物1对所测试的三种水稻病原体表现出显著的抗真菌活性，而化合物2对所测试的革兰氏阳性病原体均表现出良好的抗菌活性。
