El-Kassas Hala Yassin, El-Sheekh Mostafa M
Department of Hydrobiology, National Institute of Oceanography and Fisheries, Alexandria, Egypt E-mail :
Asian Pac J Cancer Prev. 2014;15(10):4311-7. doi: 10.7314/apjcp.2014.15.10.4311.
Nano-biotechnology is recognized as offering revolutionary changes in the field of cancer therapy and biologically synthesized gold nanoparticles are known to have a wide range of medical applications.
Gold nanoparticles (GNPs) were biosynthesized with an aqueous extract of the red alga Corallina officinalis, used as a reducing and stabilizing agent. GNPs were characterized using UV-Vis spectroscopy, transmission electron microscopy (TEM), energy dispersive analysis (EDX) and Fourier transform infra-red (FT-IR) spectroscopy and tested for cytotoxic activity against human breast cancer (MCF-7) cells cultured in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum, considering their cytotoxicty and effects on cellular DNA.
The biosynthesized GNPs were 14.6 ± 1 nm in diameter. FT-IR analysis showed that the hydroxyl functional group from polyphenols and carbonyl group from proteins could assist in formation and stabilization. The GNPs showed potent cytotoxic activity against MCF-7 cells, causing necrosis at high concentrations while lower concentrations were without effect as indicated by DNA fragmentation assay.
The antitumor activity of the biosynthesized GNPs from the red alga Corallina officinalis against human breast cancer cells may be due to the cytotoxic effects of the gold nanoparticles and the polyphenolcontent of the algal extract.
纳米生物技术被认为会给癌症治疗领域带来变革性变化，且已知生物合成的金纳米颗粒具有广泛的医学应用。
用红藻珊瑚藻的水提取物作为还原剂和稳定剂生物合成金纳米颗粒（GNPs）。使用紫外可见光谱、透射电子显微镜（TEM）、能量色散分析（EDX）和傅里叶变换红外（FT-IR）光谱对GNPs进行表征，并针对在补充有10%胎牛血清的杜氏改良 Eagle培养基中培养的人乳腺癌（MCF-7）细胞测试其细胞毒性活性，同时考虑其细胞毒性及对细胞DNA的影响。
生物合成的GNPs直径为14.6±1纳米。傅里叶变换红外光谱分析表明，多酚的羟基官能团和蛋白质的羰基官能团有助于其形成和稳定。GNPs对MCF-7细胞显示出强大的细胞毒性活性，高浓度时会导致坏死，而低浓度时无影响，DNA片段化分析表明了这一点。
红藻珊瑚藻生物合成的GNPs对人乳腺癌细胞的抗肿瘤活性可能归因于金纳米颗粒的细胞毒性作用以及藻类提取物中的多酚含量。
