Ali M A, Abul Farah M, Al-Hemaid F M, Abou-Tarboush F M
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia
Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Genet Mol Res. 2014 May 23;13(2):3981-90. doi: 10.4238/2014.May.23.9.
This study investigated the in vitro anticancer activities of a total of 14 wild angiosperms collected in Saudi Arabia. The cytotoxic activity of each extract was assessed against human breast adenocarcinoma (MCF-7) cell lines by using the MTT assay. Among the plants screened, the potential cytotoxic activity exhibited by the extract of Lavandula dentata (Lamiaceae) was identified, and we analyzed its anticancer potential by testing antiproliferative and apoptotic activity. Our results clearly show that ethanolic extract of L. dentata exhibits promising cytotoxic activity with an IC50 value of 39 μg/mL. Analysis of cell morphological changes, DNA fragmentation and apoptosis (using an Annexin V assay) also confirmed the apoptotic effect of L. dentata extract, and thus, our data call for further investigations to determine the active chemical constituent(s) and their mechanisms of inducing apoptosis.
本研究调查了在沙特阿拉伯采集的总共14种野生被子植物的体外抗癌活性。通过MTT法评估每种提取物对人乳腺腺癌（MCF-7）细胞系的细胞毒性活性。在所筛选的植物中，鉴定出了齿叶薰衣草（唇形科）提取物表现出的潜在细胞毒性活性，并且我们通过测试抗增殖和凋亡活性来分析其抗癌潜力。我们的结果清楚地表明，齿叶薰衣草乙醇提取物具有良好的细胞毒性活性，IC50值为39μg/mL。对细胞形态变化、DNA片段化和凋亡（使用膜联蛋白V检测法）的分析也证实了齿叶薰衣草提取物的凋亡作用，因此，我们的数据呼吁进一步研究以确定活性化学成分及其诱导凋亡的机制。
