Phytochemical Screening and Biological Activity of L. and Mill. Extracts.

The present study aimed to investigate the phytochemical composition of  ×  L. (MP) and Mill. (LA) extracts in terms of hydroxycinnamic acid (HCAs) content, in particular, caffeic (CA), p-cumaric (CU), ferulic (FE), and rosmarinic (RS) acids using LC-MS. Also, the antimicrobial effect against and the antiproliferative activity against two cancerous cell lines (A375 and MDA-MB-231) using the MTT assay were tested. The extracts were prepared using aromatic water which resulted from the extraction of oils from plants as extraction medium, with/without acid. The results showed that RS and FE represent the majority of HCAs compounds; the highest content of FE is found in LA (7.47 mg·gd.m.), and the maximum content of RS in MP (6.36 mg·gd.m.). Regarding the antimicrobial effect against , the two extracts showed a simulative role on the growth rate of , but a slightly inhibitory effect (69.12%) can be attributed to the acidic environment. In terms of biological activity against MDA-MB-231 breast carcinoma cell line, and A375 human melanoma cell line, at the highest employed concentration, 150 g·mL, the tested extracts present a weak antiproliferative effect.