Nikhil Vineeta, Madan Molly, Agarwal Charu, Suri Navleen
Department of Conservative Dentistry and Endodontics, Meerut, Uttar Pradesh, India.
Department of Microbiology Subharti Dental College, Meerut, Uttar Pradesh, India.
J Conserv Dent. 2014 May;17(3):271-5. doi: 10.4103/0972-0707.131795.
The purpose of this in vitro study was to determine whether the addition of 2% chlorhexidine gluconate or 10% doxycycline would enhance the antimicrobial activity of Biodentine against Staphylococcus aureus (ATCC-25923), Enterococcus faecalis (ATCC-29212), Candida albicans (ATCC-90028), and Streptococcus mutans (MTCC-497).
Three wells of 4 mm diameter and 4 mm depth on each plate were prepared on the agar medium with standardized suspensions of each microorganism. Biodentine powder mixed with 2% chlorhexidine (0.06 g) or 10% doxycycline (0.30 g) in its liquid or liquid alone was placed to fill each well. Plates were incubated at 37°C as required for microbial growth. A blinded, independent observer measured zones of inhibition. The data were analyzed using independent "t" test to compare the differences among the three cement preparations for different micro-organisms.
All Biodentine samples inhibited microbial growth. The highest mean diameters of zone of inhibition for all the micro-organisms were found around Biodentine/chlorhexidine (13.417) followed by Biodentine alone (12.236) and Biodentine/doxycycline (11.25).
In conclusion, adding 2% chlorhexidine gluconate in liquid of Biodentine enhanced the antimicrobial activity of Biodentine against all the tested micro-organisms except Candida albicans, while addition of 10% doxycycline decreased the antimicrobial activity of Biodentine. The differences were significant statistically (P < 0.05).
本体外研究旨在确定添加2%葡萄糖酸氯己定或10%强力霉素是否会增强生物陶瓷（Biodentine）对金黄色葡萄球菌（ATCC - 25923）、粪肠球菌（ATCC - 29212）、白色念珠菌（ATCC - 90028）和变形链球菌（MTCC - 497）的抗菌活性。
在含有每种微生物标准化悬液的琼脂培养基上，在每个平板上制备3个直径4 mm、深度4 mm的孔。将与2%氯己定（0.06 g）或10%强力霉素（0.30 g）混合的生物陶瓷粉末或仅其液体放入每个孔中。根据微生物生长要求，将平板在37°C下孵育。由一位不知情的独立观察者测量抑菌圈。使用独立“t”检验分析数据，以比较三种不同微生物的粘结剂制剂之间的差异。
所有生物陶瓷样品均抑制了微生物生长。在所有微生物中，抑菌圈平均直径最大的是生物陶瓷/氯己定周围（13.417），其次是单独的生物陶瓷（12.236）和生物陶瓷/强力霉素（11.25）。
总之，在生物陶瓷液体中添加2%葡萄糖酸氯己定可增强生物陶瓷对除白色念珠菌外的所有测试微生物的抗菌活性，而添加10%强力霉素则降低了生物陶瓷的抗菌活性。差异具有统计学意义（P < 0.05）。
